Production of a Solvent, Detergent, and Thermotolerant Lipase by a Newly Isolated Acinetobacter sp. in Submerged and Solid-State Fermentations
Joint Authors
Beh, Boon Kee
Ebrahimpour, Afshin
Lai, Oi Ming
Khoramnia, Anahita
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-27
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The lipase production ability of a newly isolated Acinetobacter sp.
in submerged (SmF) and solid-state (SSF) fermentations was evaluated.
The results demonstrated this strain as one of the rare bacterium, which is able to grow and produce lipase in SSF even more than SmF.
Coconut oil cake as a cheap agroindustrial residue was employed as the solid substrate.
The lipase production was optimized in both media using artificial neural network.
Multilayer normal and full feed forward backpropagation networks were selected to build predictive models to optimize the culture parameters for lipase production in SmF and SSF systems, respectively.
The produced models for both systems showed high predictive accuracy where the obtained conditions were close together.
The produced enzyme was characterized as a thermotolerant lipase, although the organism was mesophile.
The optimum temperature for the enzyme activity was 45°C where 63% of its activity remained at 70°C after 2 h.
This lipase remained active after 24 h in a broad range of pH (6–11).
The lipase demonstrated strong solvent and detergent tolerance potentials.
Therefore, this inexpensive lipase production for such a potent and industrially valuable lipase is promising and of considerable commercial interest for biotechnological applications.
American Psychological Association (APA)
Khoramnia, Anahita& Ebrahimpour, Afshin& Beh, Boon Kee& Lai, Oi Ming. 2011. Production of a Solvent, Detergent, and Thermotolerant Lipase by a Newly Isolated Acinetobacter sp. in Submerged and Solid-State Fermentations. BioMed Research International،Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-990254
Modern Language Association (MLA)
Khoramnia, Anahita…[et al.]. Production of a Solvent, Detergent, and Thermotolerant Lipase by a Newly Isolated Acinetobacter sp. in Submerged and Solid-State Fermentations. BioMed Research International No. 2011 (2011), pp.1-12.
https://search.emarefa.net/detail/BIM-990254
American Medical Association (AMA)
Khoramnia, Anahita& Ebrahimpour, Afshin& Beh, Boon Kee& Lai, Oi Ming. Production of a Solvent, Detergent, and Thermotolerant Lipase by a Newly Isolated Acinetobacter sp. in Submerged and Solid-State Fermentations. BioMed Research International. 2011. Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-990254
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990254