In Vivo Imaging of Particle-Induced Inflammation and Osteolysis in the Calvariae of NFκBLuciferase Transgenic Mice
Joint Authors
Onodera, Shin
Tohyama, Harukazu
Honma, Ken-ichi
Yasuda, Kazunori
Kwon, Hyuck Joon
Takahashi, Kunihiko
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-09-06
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Wear debris causes biological response which can result in periprosthetic osteolysis after total joint replacement surgery.
Nuclear factor-kappa B (NFκB), a representative transcription factor involved in inflammation, is believed to play an important role in this event by regulating the production of proinflammatory mediators and osteoclastogenesis.
In this study, we sought to determine whether activation of NFκB in response to stimulation by particles could be visualized by in vivo imaging.
We loaded polyethylene (PE) particles onto the calvaria of NFκB/luciferase transgenic mouse, and detected luminescence generated by activation of NFκB.
On day 7 after loading, the level of luminescence was maximal.
Levels of luminescence were significantly correlated with the levels of luciferase activity, proinflammatory mediator mRNAs, and bone resorption parameters.
This system, which enabled us to evaluate particle-induced inflammation and osteolysis without sacrificing mice, constitutes a useful tool for evaluating the efficacy of prophylaxis or treatments for particle-induced osteolysis.
American Psychological Association (APA)
Takahashi, Kunihiko& Onodera, Shin& Tohyama, Harukazu& Kwon, Hyuck Joon& Honma, Ken-ichi& Yasuda, Kazunori. 2010. In Vivo Imaging of Particle-Induced Inflammation and Osteolysis in the Calvariae of NFκBLuciferase Transgenic Mice. BioMed Research International،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-990264
Modern Language Association (MLA)
Takahashi, Kunihiko…[et al.]. In Vivo Imaging of Particle-Induced Inflammation and Osteolysis in the Calvariae of NFκBLuciferase Transgenic Mice. BioMed Research International No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-990264
American Medical Association (AMA)
Takahashi, Kunihiko& Onodera, Shin& Tohyama, Harukazu& Kwon, Hyuck Joon& Honma, Ken-ichi& Yasuda, Kazunori. In Vivo Imaging of Particle-Induced Inflammation and Osteolysis in the Calvariae of NFκBLuciferase Transgenic Mice. BioMed Research International. 2010. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-990264
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990264