Sperm DNA Integrity and Meiotic Behavior Assessment in an Infertile Male Carrier of a 9qh+++ Polymorphism
Joint Authors
Benet, Jordi
Amengual, María J.
Guitart, Miriam
García-Peiró, Agustín
Oliver-Bonet, M.
Abad, C.
Navarro, J.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-11-04
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Although several reports on male infertility suggest a relationship between chromosome 9 polymorphisms and infertility, the effects on the phenotype have not been extensively reported.
In this study, an infertile patient was found to carry a 9qh+++ chromosome.
The flow cytometric TUNEL assay and SCD test have been applied to characterize sperm DNA integrity.
In order to assess its meiotic behaviour, synapsis, recombination, and aneuploidy, analyses have been also performed.
Sperm DNA fragmentation (SDF) was 77.81% and 87% for the TUNEL and SCD tests, respectively.
Ninety-two percent of pachytene cells analyzed showed meiotic abnormalities.
The mean number of MLH1 foci per pachytene in the control group was higher (49) than the mean found in the 9qh+++ patient (38) (P<.0001).
In spermatozoa, significant increases of disomy rates were observed for chromosome 18 and for the sex chromosomes (P<.0001).
These disturbances could be present in other male carriers of a less marked 9qh+.
American Psychological Association (APA)
García-Peiró, Agustín& Oliver-Bonet, M.& Navarro, J.& Abad, C.& Guitart, Miriam& Amengual, María J.…[et al.]. 2010. Sperm DNA Integrity and Meiotic Behavior Assessment in an Infertile Male Carrier of a 9qh+++ Polymorphism. BioMed Research International،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-990267
Modern Language Association (MLA)
García-Peiró, Agustín…[et al.]. Sperm DNA Integrity and Meiotic Behavior Assessment in an Infertile Male Carrier of a 9qh+++ Polymorphism. BioMed Research International No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-990267
American Medical Association (AMA)
García-Peiró, Agustín& Oliver-Bonet, M.& Navarro, J.& Abad, C.& Guitart, Miriam& Amengual, María J.…[et al.]. Sperm DNA Integrity and Meiotic Behavior Assessment in an Infertile Male Carrier of a 9qh+++ Polymorphism. BioMed Research International. 2010. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-990267
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990267