Synergistic Antitumor Effect of Dichloroacetate in Combination with 5-Fluorouracil in Colorectal Cancer
Joint Authors
Liang, Houjie
Li, Jianjun
Tong, Jingtao
Xie, Ganfeng
Pan, Feng
He, Jinxia
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-01-24
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Dichloroacetate (DCA), an inhibitor of pyruvate dehydrogenase kinase (PDK), has been recently demonstrated as a promising nontoxic antineoplastic agent that promotes apoptosis of cancer cells.
In the present study, we aimed to investigate the antitumor effect of DCA combined with 5-Fluorouracil (5-FU) on colorectal cancer (CRC) cells.
Four human CRC cell lines were treated with DCA or 5-FU, or a combination of DCA and 5-FU.
The cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
The interaction between DCA and 5-FU was evaluated by the median effect principle.
Immunocytochemistry with bromodeoxyuridine (BrdU) was carried out to determine the proliferation of CRC cells.
Cell cycle and apoptosis were measured by flow cytometry, and the expression of apoptosis-related molecules was assessed by western blot.
Our results demonstrated that DCA inhibited the viability of CRC cells and had synergistic antiproliferation in combination with 5-FU.
Moreover, compared with 5-FU alone, the apoptosis of CRC cells treated with DCA and 5-FU was enhanced and demonstrated with the changes of Bcl-2, Bax, and caspase-3 proteins.
Our results suggest that DCA has a synergistic antitumor effect with 5-FU on CRC cell lines in vitro.
American Psychological Association (APA)
Tong, Jingtao& Xie, Ganfeng& He, Jinxia& Li, Jianjun& Pan, Feng& Liang, Houjie. 2011. Synergistic Antitumor Effect of Dichloroacetate in Combination with 5-Fluorouracil in Colorectal Cancer. BioMed Research International،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-990272
Modern Language Association (MLA)
Tong, Jingtao…[et al.]. Synergistic Antitumor Effect of Dichloroacetate in Combination with 5-Fluorouracil in Colorectal Cancer. BioMed Research International No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-990272
American Medical Association (AMA)
Tong, Jingtao& Xie, Ganfeng& He, Jinxia& Li, Jianjun& Pan, Feng& Liang, Houjie. Synergistic Antitumor Effect of Dichloroacetate in Combination with 5-Fluorouracil in Colorectal Cancer. BioMed Research International. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-990272
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990272