Magnetic Resonance Microscopy Contribution to Interpret High-Resolution Magic Angle Spinning Metabolomic Data of Human Tumor Tissue
Joint Authors
Martínez-Granados, Beatriz
Esteve, Vicent
Martínez-Bisbal, M. Carmen
Celda, Bernardo
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-08-16
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
HRMAS NMR is considered a valuable technique to obtain detailed metabolic profile of unprocessed tissues.
To properly interpret the HRMAS metabolomic results, detailed information of the actual state of the sample inside the rotor is needed.
MRM (Magnetic Resonance Microscopy) was applied for obtaining structural and spatially localized metabolic information of the samples inside the HRMAS rotors.
The tissue was observed stuck to the rotor wall under the effect of HRMAS spinning.
MRM spectroscopy showed a transference of metabolites from the tissue to the medium.
The sample shape and the metabolite transfer after HRMAS indicated that tissue had undergone alterations and it can not be strictly considered as intact.
This must be considered when HRMAS is used for metabolic tissue characterization, and it is expected to be highly dependent on the manipulation of the sample.
The localized spectroscopic information of MRM reveals the biochemical compartmentalization on tissue samples hidden in the HRMAS spectrum.
American Psychological Association (APA)
Martínez-Bisbal, M. Carmen& Esteve, Vicent& Martínez-Granados, Beatriz& Celda, Bernardo. 2010. Magnetic Resonance Microscopy Contribution to Interpret High-Resolution Magic Angle Spinning Metabolomic Data of Human Tumor Tissue. BioMed Research International،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-990279
Modern Language Association (MLA)
Martínez-Bisbal, M. Carmen…[et al.]. Magnetic Resonance Microscopy Contribution to Interpret High-Resolution Magic Angle Spinning Metabolomic Data of Human Tumor Tissue. BioMed Research International No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-990279
American Medical Association (AMA)
Martínez-Bisbal, M. Carmen& Esteve, Vicent& Martínez-Granados, Beatriz& Celda, Bernardo. Magnetic Resonance Microscopy Contribution to Interpret High-Resolution Magic Angle Spinning Metabolomic Data of Human Tumor Tissue. BioMed Research International. 2010. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-990279
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990279