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Mutation of Herpesvirus Saimiri ORF51 Glycoprotein Specifically Targets Infectivity to Hepatocellular Carcinoma Cell Lines
Joint Authors
Whitehouse, Adrian
Turrell, Susan J.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-10-26
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Herpesvirus saimiri (HVS) is a gamma herpesvirus with several properties that make it an amenable gene therapy vector; namely its large packaging capacity, its ability to persist as a nonintegrated episome, and its ability to infect numerous human cell types.
We used RecA-mediated recombination to develop an HVS vector with a mutated virion protein.
The heparan sulphate-binding region of HVS ORF51 was substituted for a peptide sequence which interacts with somatostatin receptors (SSTRs), overexpressed on hepatocellular carcinoma (HCC) cells.
HVS mORF51 showed reduced infectivity in non-HCC human cell lines compared to wild-type virus.
Strikingly, HVS mORF51 retained its ability to infect HCC cell lines efficiently.
However, neutralisation assays suggest that HVS mORF51 has no enhanced binding to SSTRs.
Therefore, mutation of the ORF51 glycoprotein has specifically targeted HVS to HCC cell lines by reducing the infectivity of other cell types; however, the mechanism for this targeting is unknown.
American Psychological Association (APA)
Turrell, Susan J.& Whitehouse, Adrian. 2010. Mutation of Herpesvirus Saimiri ORF51 Glycoprotein Specifically Targets Infectivity to Hepatocellular Carcinoma Cell Lines. BioMed Research International،Vol. 2011, no. 2011, pp.1-14.
https://search.emarefa.net/detail/BIM-990284
Modern Language Association (MLA)
Turrell, Susan J.& Whitehouse, Adrian. Mutation of Herpesvirus Saimiri ORF51 Glycoprotein Specifically Targets Infectivity to Hepatocellular Carcinoma Cell Lines. BioMed Research International No. 2011 (2011), pp.1-14.
https://search.emarefa.net/detail/BIM-990284
American Medical Association (AMA)
Turrell, Susan J.& Whitehouse, Adrian. Mutation of Herpesvirus Saimiri ORF51 Glycoprotein Specifically Targets Infectivity to Hepatocellular Carcinoma Cell Lines. BioMed Research International. 2010. Vol. 2011, no. 2011, pp.1-14.
https://search.emarefa.net/detail/BIM-990284
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990284