Induction and Flow Cytometry Identification of Tetraploids from Seed-Derived Explants through Colchicine Treatments in Catharanthus roseus (L.) G. Don
Joint Authors
Wang, Guo-Feng
Guo, Xin-Bo
Zhao, Jing-Ya
Liu, Pin
Pan, Qi-Fang
Xing, Shi-Hai
Tian, Yue-Sheng
Wang, Quan
Sun, Xiao-Fen
Tang, Ke-Xuan
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-03
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The tetraploid plants of Catharanthus roseus (L.) G.
Don was obtained by colchicine induction from seeds explants, and the ploidy of the plants was identified by flow cytometry.
The optimal treatment is 0.2% colchicine solution treated for 24 hours, and the induction rate reaches up to 30%.
Comparing with morphological characteristics and growth habits between tetraploids and the control, we found that tetraploids of C.
roseus had larger stoma and more branches and leaves.
HPLC analysis showed tetraploidization could increase the contents of terpenoid indole alkaloids in C.
roseus.
Thus, tetraploidization could be used to produce higher alkaloids lines for commercial use.
QRT-PCR results showed that the expression of enzymes involved in terpenoid indole alkaloids biosynthesis pathway had increased in the tetraploid plants.
To our knowledge, this was the first paper to explore the secondary metabolism in autotetraploid C.
roseus induced by colchicine.
American Psychological Association (APA)
Xing, Shi-Hai& Guo, Xin-Bo& Wang, Quan& Pan, Qi-Fang& Tian, Yue-Sheng& Liu, Pin…[et al.]. 2011. Induction and Flow Cytometry Identification of Tetraploids from Seed-Derived Explants through Colchicine Treatments in Catharanthus roseus (L.) G. Don. BioMed Research International،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-990289
Modern Language Association (MLA)
Xing, Shi-Hai…[et al.]. Induction and Flow Cytometry Identification of Tetraploids from Seed-Derived Explants through Colchicine Treatments in Catharanthus roseus (L.) G. Don. BioMed Research International No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-990289
American Medical Association (AMA)
Xing, Shi-Hai& Guo, Xin-Bo& Wang, Quan& Pan, Qi-Fang& Tian, Yue-Sheng& Liu, Pin…[et al.]. Induction and Flow Cytometry Identification of Tetraploids from Seed-Derived Explants through Colchicine Treatments in Catharanthus roseus (L.) G. Don. BioMed Research International. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-990289
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990289