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Identification of Urinary Biomarkers of Colon Inflammation in IL10-- Mice Using Short-Column LCMS Metabolomics
Joint Authors
Lane, Geoff
Fraser, Karl
Lin, Hui-Ming
Rowan, Daryl
Otter, Don
Edmunds, Shelley
Cao, Mingshu
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-10-28
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The interleukin-10-deficient (IL10-/-) mouse develops colon inflammation in response to normal intestinal microflora and has been used as a model of Crohn's disease.
Short-Column LCMS metabolite profiling of urine from IL10-/- and wild-type (WT) mice was used, in two independent experiments, to identify mass spectral ions differing in intensity between these two genotypes.
Three differential metabolites were identified as xanthurenic acid and as the glucuronides of xanthurenic acid and of α-CEHC (2,5,7,8-tetramethyl-2-(2′-carboxyethyl)-6-hydroxychroman).
The significance of several differential metabolites as potential biomarkers of colon inflammation was evaluated in an experiment which compared metabolite concentrations in IL10-/- and WT mice housed, either under conventional conditions and dosed with intestinal microflora, or maintained under specific pathogen-free (SPF) conditions.
Concentrations of xanthurenic acid, α-CEHC glucuronide, and an unidentified metabolite m/z 495-/497+ were associated with the degree of inflammation in IL10-/- mice and may prove useful as biomarkers of colon inflammation.
American Psychological Association (APA)
Otter, Don& Cao, Mingshu& Lin, Hui-Ming& Fraser, Karl& Edmunds, Shelley& Lane, Geoff…[et al.]. 2010. Identification of Urinary Biomarkers of Colon Inflammation in IL10-- Mice Using Short-Column LCMS Metabolomics. BioMed Research International،Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-990435
Modern Language Association (MLA)
Otter, Don…[et al.]. Identification of Urinary Biomarkers of Colon Inflammation in IL10-- Mice Using Short-Column LCMS Metabolomics. BioMed Research International No. 2011 (2011), pp.1-12.
https://search.emarefa.net/detail/BIM-990435
American Medical Association (AMA)
Otter, Don& Cao, Mingshu& Lin, Hui-Ming& Fraser, Karl& Edmunds, Shelley& Lane, Geoff…[et al.]. Identification of Urinary Biomarkers of Colon Inflammation in IL10-- Mice Using Short-Column LCMS Metabolomics. BioMed Research International. 2010. Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-990435
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990435