Factors Affecting Splicing Strength of Yeast Genes
Joint Authors
Source
International Journal of Genomics
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-11-20
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Accurate and efficient splicing is of crucial importance for highly-transcribed intron-containing genes (ICGs) in rapidly replicating unicellular eukaryotes such as the budding yeast Saccharomyces cerevisiae.
We characterize the 5′ and 3′ splice sites (ss) by position weight matrix scores (PWMSs), which is the highest for the consensus sequence and the lowest for splice sites differing most from the consensus sequence and used PWMS as a proxy for splicing strength.
HAC1, which is known to be spliced by a nonspliceosomal mechanism, has the most negative PWMS for both its 5′ ss and 3′ ss.
Several genes under strong splicing regulation and requiring additional splicing factors for their splicing also have small or negative PWMS values.
Splicing strength is higher for highly transcribed ICGs than for lowly transcribed ICGs and higher for transcripts that bind strongly to spliceosomes than those that bind weakly.
The 3′ splice site features a prominent poly-U tract before the 3′AG.
Our results suggest the potential of using PWMS as a screening tool for ICGs that are either spliced by a nonspliceosome mechanism or under strong splicing regulation in yeast and other fungal species.
American Psychological Association (APA)
Ma, Pinchao& Xia, Xuhua. 2011. Factors Affecting Splicing Strength of Yeast Genes. International Journal of Genomics،Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-990473
Modern Language Association (MLA)
Ma, Pinchao& Xia, Xuhua. Factors Affecting Splicing Strength of Yeast Genes. International Journal of Genomics No. 2011 (2011), pp.1-13.
https://search.emarefa.net/detail/BIM-990473
American Medical Association (AMA)
Ma, Pinchao& Xia, Xuhua. Factors Affecting Splicing Strength of Yeast Genes. International Journal of Genomics. 2011. Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-990473
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990473