Molecular and Biological Characterization of Chinese Sacbrood Virus LN Isolate
Joint Authors
Song, Yang
Jian, Cheng
Shude, Wang
Mingxiao, Ma
Ming, Li
Pengfei, Li
Source
International Journal of Genomics
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-02-10
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Chinese sacbrood virus (CSBV) was purified from diseased insects, and its genome was cloned and sequenced.
The genomic RNA of CSBV is 8863 nucleotides in length and contains a single large open reading frame encoding a 319.614 kDa polyprotein.
The coding sequence is flanked by a 178-nucleotide 5′ nontranslated leader sequence and a 142-nucleotide 3′ nontranslated region, followed a poly(A) tail.
Four major structural proteins, VP1,VP2, VP3 and VP4, were predicted in the N-teminal of the polyprotein.
The C-terminal part of the polyprotein contains sequence motifs which is a typical and well-characterized picornavirus nonstructural proteins: an RNA helicase, a chymotrypsin-like 3C protease, and an RNA-dependent RNA polymerase.
Genetic analysis shows that the CSBV-LN had a 13-amino-acid deletion at amino acid positions 710–719 and 727–729 in comparison with CSBV-GZ and SBV-UK, and the SBV-UK had a 7-amino-acid deletion at amino acid positions 2124–2132 in comparison with CSBV-GZ and CSBV-LN, and the CSBV-GZ and CSBV-LN had a 6-amino-acid deletion at amino acid positions 2143–2150 in comparison with SBV-UK.
Phylogenetic analysis using RdRp of selected picorna-like viruses shows that CSBV/SBV and Deformed Wing Virus (DWV) tend to group together, which possesses an RNA of similar size and gene order.
American Psychological Association (APA)
Mingxiao, Ma& Ming, Li& Jian, Cheng& Song, Yang& Shude, Wang& Pengfei, Li. 2011. Molecular and Biological Characterization of Chinese Sacbrood Virus LN Isolate. International Journal of Genomics،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-990480
Modern Language Association (MLA)
Mingxiao, Ma…[et al.]. Molecular and Biological Characterization of Chinese Sacbrood Virus LN Isolate. International Journal of Genomics No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-990480
American Medical Association (AMA)
Mingxiao, Ma& Ming, Li& Jian, Cheng& Song, Yang& Shude, Wang& Pengfei, Li. Molecular and Biological Characterization of Chinese Sacbrood Virus LN Isolate. International Journal of Genomics. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-990480
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990480