Differential Protein Expression in Sugarcane during Sugarcane-Sporisorium scitamineum Interaction Revealed by 2-DE and MALDI-TOF-TOFMS

Abstract EN

To understand the molecular basis of a specific plant-pathogen interaction, it is important to identify plant proteins that respond to the pathogen attack.

Two sugarcane varieties, NCo376 and Ya71-374, were used in this study.

By applying 2-dimensional electrophoresis (2-DE), the protein expression profile of sugarcane after inoculating with Sporisorium scitamineum was analyzed.

In total, 23 differentially expressed proteins were identified by MALDI-TOF-TOF/MS.

Bioinformatics analysis revealed that the functions of these 20 differential proteins were associated with such functions as photosynthesis, signal transduction, and disease resistance, while the function of the remaining three proteins was not determined.

From above, we can assume that the protein regulatory network during the interaction between sugarcane and S.

scitamineum is complicated.

This represents the first proteomic investigation focused on highlighting the alterations of the protein expression profile in sugarcane exposed to S.

scitamineum, and it provides reference information on sugarcane response to S.

scitamineum stress at the protein level.