A High-Performance Liquid Chromatography:Chemiluminescence Method for Potential Determination of Vardenafil in Dietary Supplement
Joint Authors
Zhao, Min
Lv, Jiagen
Nie, Yingchun
Wang, Fei
Di, Youjun
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-01-02
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
A flow method of high-performance liquid chromatography (HPLC) seperation and chemiluminescence (CL) detection for sensitive vardenafil analysis in dietary supplements was developed.
The vardenafil separation was achieved on a C18 column at 30∘C using ethanol-H3PO4 and ethylenediaminetetraacetic acid disodium salt (Na2EDTA) aqueous solution (25 : 75, v/v%) as mobile phase.
The followed continuous CL detection was conducted based on the strong CL enhancement by the presence of vardenafil to luminol-K3Fe(CN)6 reaction in alkaline medium.
At the flow rate of 0.8 mL/min, the vardenafil retention time (tR) was 6.4 min.
Factors that affected the HPLC resolution and CL detection were studied and optimized.
The calibration curve obtained for vardenafil standard was linear in concentration range of 8.0 × 10−7~1.0 × 10−4 mol/L.
The relative standard deviations (RSD) of intraday and interday precision were less than 3.5%.
The proposed method was applied to the vardenafil determination in oral liquid, wine, and capsule samples.
American Psychological Association (APA)
Di, Youjun& Zhao, Min& Nie, Yingchun& Wang, Fei& Lv, Jiagen. 2011. A High-Performance Liquid Chromatography:Chemiluminescence Method for Potential Determination of Vardenafil in Dietary Supplement. Journal of Analytical Methods in Chemistry،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-990614
Modern Language Association (MLA)
Di, Youjun…[et al.]. A High-Performance Liquid Chromatography:Chemiluminescence Method for Potential Determination of Vardenafil in Dietary Supplement. Journal of Analytical Methods in Chemistry No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-990614
American Medical Association (AMA)
Di, Youjun& Zhao, Min& Nie, Yingchun& Wang, Fei& Lv, Jiagen. A High-Performance Liquid Chromatography:Chemiluminescence Method for Potential Determination of Vardenafil in Dietary Supplement. Journal of Analytical Methods in Chemistry. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-990614
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990614