Studies on bacteria associated with respiratory disorder in poultry with special reference to pasteurella multocida

Abstract EN

Trials for isolation of Pasteurella multocida from lung samples of chickens suffering from Pneumonia were curried out.

The identity of Pasteurella multocida was proved by mouse inoculation and by polymerase chain reaction (PCR).

120 samples with pneumonia were collected from 500 slaughtered chickens with history of respiratory disorders.

All samples inoculated into blood agar supplemented with 10% sheep blood.

The bacteriological examination was carried out on all samples and revealed that the incidence of P.

multocida was 6 (5%).

In addition, mouse inoculation test was carried out on P.

multocida suspected isolates.

Five (83.3%) of isolates were all positive and one (16.3%) isolate not lethal for mice.

All P.

multocida strains that were positive by culture were also detected to be positive by the PCR.

Toxigenic P.

multocida was not isolated from any of these specimens .