Assessment of Glucagon-Like Peptide-1 Analogue and Renin Inhibitor on the Binding and Regulation of GLP-1 Receptor in Type 1 Diabetic Rat Hearts
Joint Authors
Bikhazi, Anwar B.
Zwainy, Nadine S.
al-Lafi, Sawsan M.
Bitar, Khalil M.
Artinian, Shushan B.
Boutary, Suzan S.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-06-04
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
This study focuses on the effects of long-term renin-angiotensin system suppression and/or incretin mimetic therapies on the regulation and binding affinity of GLP-1 to its receptor in the coronary endothelium (CE) and cardiomyocytes (CMs) of type 1 diabetic male Sprague-Dawley rats.
The groups assessed are normal (N), streptozotocin-induced diabetic (D), Insulin treated (DI), Exendin-4 treated (DE), Aliskiren treated (DA), cotreated with Insulin and Aliskiren (DIA) and cotreated with exendin-4 and Aliskiren (DEA).
Heart perfusion with 125I-GLP-1 was performed to estimate GLP-1 binding affinity (τ=1/k-n) to its receptor in the heart.
Western Blotting was assessed to determine the expression variation of GLP-1 receptor in the heart.
Plasma GLP-1 levels were measured using Enzyme-Linked Immunosorbent Assay (ELISA).
Diabetes decreased the τ value on CE and increased it on CMs compared to normal.
The combination of Exendin-4 with Aliskiren showed a normalizing effect on the binding affinity of GLP-1 at the coronary endothelium, while at the cardiomyocyte level Exendin-4 treatment alone was the most effective.
American Psychological Association (APA)
Artinian, Shushan B.& al-Lafi, Sawsan M.& Boutary, Suzan S.& Bitar, Khalil M.& Zwainy, Nadine S.& Bikhazi, Anwar B.. 2011. Assessment of Glucagon-Like Peptide-1 Analogue and Renin Inhibitor on the Binding and Regulation of GLP-1 Receptor in Type 1 Diabetic Rat Hearts. Journal of Diabetes Research،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-990640
Modern Language Association (MLA)
Artinian, Shushan B.…[et al.]. Assessment of Glucagon-Like Peptide-1 Analogue and Renin Inhibitor on the Binding and Regulation of GLP-1 Receptor in Type 1 Diabetic Rat Hearts. Journal of Diabetes Research No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-990640
American Medical Association (AMA)
Artinian, Shushan B.& al-Lafi, Sawsan M.& Boutary, Suzan S.& Bitar, Khalil M.& Zwainy, Nadine S.& Bikhazi, Anwar B.. Assessment of Glucagon-Like Peptide-1 Analogue and Renin Inhibitor on the Binding and Regulation of GLP-1 Receptor in Type 1 Diabetic Rat Hearts. Journal of Diabetes Research. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-990640
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990640