Altered Expression of Somatostatin Receptors in Pancreatic Islets from NOD Mice Cultured at Different Glucose Concentrations In Vitro and in Islets Transplanted to Diabetic NOD Mice In Vivo

Joint Authors

Ludvigsen, Eva
Stridsberg, Mats
Sandler, Stellan
Janson, Eva T.

Source

Journal of Diabetes Research

Issue

Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2011-09-05

Country of Publication

Egypt

No. of Pages

8

Main Subjects

Diseases
Medicine

Abstract EN

Somatostatin acts via five receptors (sst1-5).

We investigated if the changes in pancreatic islet sst expression in diabetic NOD mice compared to normoglycemic mice are a consequence of hyperglycemia or the ongoing immune reaction in the pancreas.

Pancreatic islets were isolated from NOD mice precultured for 5 days and further cultured for 3 days at high or low glucose before examined.

Islets were also isolated from NOD mice and transplanted to normal or diabetic mice in a number not sufficient to cure hyperglycemia.

After three days, the transplants were removed and stained for sst1-5 and islet hormones.

Overall, changes in sst islet cell expression were more common in islets cultured in high glucose concentration in vitro as compared to the islet transplantation in vivo to diabetic mice.

The beta and PP cells exhibited more frequent changes in sst expression, while the alpha and delta cells were relatively unaffected by the high glucose condition.

Our findings suggest that the glucose level may alter sst expressed in islets cells; however, immune mechanisms may counteract such changes in islet sst expression.

American Psychological Association (APA)

Ludvigsen, Eva& Stridsberg, Mats& Janson, Eva T.& Sandler, Stellan. 2011. Altered Expression of Somatostatin Receptors in Pancreatic Islets from NOD Mice Cultured at Different Glucose Concentrations In Vitro and in Islets Transplanted to Diabetic NOD Mice In Vivo. Journal of Diabetes Research،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-990665

Modern Language Association (MLA)

Ludvigsen, Eva…[et al.]. Altered Expression of Somatostatin Receptors in Pancreatic Islets from NOD Mice Cultured at Different Glucose Concentrations In Vitro and in Islets Transplanted to Diabetic NOD Mice In Vivo. Journal of Diabetes Research No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-990665

American Medical Association (AMA)

Ludvigsen, Eva& Stridsberg, Mats& Janson, Eva T.& Sandler, Stellan. Altered Expression of Somatostatin Receptors in Pancreatic Islets from NOD Mice Cultured at Different Glucose Concentrations In Vitro and in Islets Transplanted to Diabetic NOD Mice In Vivo. Journal of Diabetes Research. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-990665

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-990665