Development of Type 1 Diabetes Mellitus in Nonobese Diabetic Mice Follows Changes in Thymocyte and Peripheral T Lymphocyte Transcriptional Activity
Joint Authors
Fornari, Thais A.
Passos, Geraldo A.
Macedo, Claudia
Sakamoto-Hojo, Elza Tiemi
Donate, Paula B.
Donadi, Eduardo Antônio
Source
Journal of Immunology Research
Issue
Vol. 2011, Issue 2011 (31 Dec. 2010), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-06-15
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
As early as one month of age, nonobese diabetic (NOD) mice feature pancreatic infiltration of autoreactive T lymphocytes, which destruct insulin-producing beta cells, producing autoimmune diabetes mellitus (T1D) within eight months.
Thus, we hypothesized that during the development of T1D, the transcriptional modulation of immune reactivity genes may occur as thymocytes mature into peripheral T lymphocytes.
The transcriptome of thymocytes and peripheral CD3+ T lymphocytes from prediabetic or diabetic mice analyzed through microarray hybridizations identified 2,771 differentially expressed genes.
Hierarchical clustering grouped mice according to age/T1D onset and genes according to their transcription profiling.
The transcriptional activity of thymocytes developing into peripheral T lymphocytes revealed sequential participation of genes involved with CD4+/CD8+ T-cell differentiation (Themis), tolerance induction by Tregs (Foxp3), and apoptosis (Fasl) soon after T-cell activation (IL4), while the emergence of T1D coincided with the expression of cytotoxicity (Crtam) and inflammatory response genes (Tlr) by peripheral T lymphocytes.
American Psychological Association (APA)
Fornari, Thais A.& Donate, Paula B.& Macedo, Claudia& Sakamoto-Hojo, Elza Tiemi& Donadi, Eduardo Antônio& Passos, Geraldo A.. 2011. Development of Type 1 Diabetes Mellitus in Nonobese Diabetic Mice Follows Changes in Thymocyte and Peripheral T Lymphocyte Transcriptional Activity. Journal of Immunology Research،Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-990727
Modern Language Association (MLA)
Fornari, Thais A.…[et al.]. Development of Type 1 Diabetes Mellitus in Nonobese Diabetic Mice Follows Changes in Thymocyte and Peripheral T Lymphocyte Transcriptional Activity. Journal of Immunology Research Vol. 2011, no. 2011 (2010), pp.1-12.
https://search.emarefa.net/detail/BIM-990727
American Medical Association (AMA)
Fornari, Thais A.& Donate, Paula B.& Macedo, Claudia& Sakamoto-Hojo, Elza Tiemi& Donadi, Eduardo Antônio& Passos, Geraldo A.. Development of Type 1 Diabetes Mellitus in Nonobese Diabetic Mice Follows Changes in Thymocyte and Peripheral T Lymphocyte Transcriptional Activity. Journal of Immunology Research. 2011. Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-990727
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990727