Phenotype and Function of CD25-Expressing B Lymphocytes Isolated from Human Umbilical Cord Blood
Joint Authors
Source
Journal of Immunology Research
Issue
Vol. 2011, Issue 2011 (31 Dec. 2010), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-18
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
We have shown that approximately 30% of human peripheral blood B-cells express CD25.
B cells expressing CD25 display a mature phenotype belonging to the memory B-cell population and have a better proliferative and antigen-presenting capacity.
The aim of the present study was to characterize the CD25-expressing subset of B cells in human cord blood.
Material and Methods.
Mononuclear cell fraction from human cord blood (n=34) and peripheral adult blood (n=22) was sorted into CD20+CD25+ and CD20+CD25- B-cell populations.
Phenotype and function of these B-cell populations were compared using flow cytometry, proliferation, cytokine production, and immunoglobulin secretion.
Results.
CD25-expressing B cells are a limited population of cord blood mononuclear cells representing 5% of the CD20+ B cells.
They are characterised by high expression of CD5 in cord blood and CD27 in adult blood.
CD25-expressing B cells express a functional IL-2 receptor and high levels of CC-chemokine receptors and spontaneously produce antibodies of IgG and IgM subclass.
Conclusions.
CD25 expression is a common denominator of a specific immunomodulatory B-cell subset ready to proliferate upon IL-2 stimulation, possibly ready to migrate and home into the peripheral tissue for further differentiation/action.
American Psychological Association (APA)
Amu, Sylvie& Brisslert, Mikael. 2011. Phenotype and Function of CD25-Expressing B Lymphocytes Isolated from Human Umbilical Cord Blood. Journal of Immunology Research،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-990802
Modern Language Association (MLA)
Amu, Sylvie& Brisslert, Mikael. Phenotype and Function of CD25-Expressing B Lymphocytes Isolated from Human Umbilical Cord Blood. Journal of Immunology Research Vol. 2011, no. 2011 (2010), pp.1-9.
https://search.emarefa.net/detail/BIM-990802
American Medical Association (AMA)
Amu, Sylvie& Brisslert, Mikael. Phenotype and Function of CD25-Expressing B Lymphocytes Isolated from Human Umbilical Cord Blood. Journal of Immunology Research. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-990802
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990802