Evaluation of Epstein-Barr Virus Latent Membrane Protein 2 Specific T-Cell Receptors Driven by T-Cell Specific Promoters Using Lentiviral Vector
Joint Authors
Sun, Hua
Hou, Jiajie
Chen, Yun
Yang, Dongchang
Yao, Kun
Mu, Xiaoxin
Sun, Beicheng
Shao, Qing
Gao, Yun
Jiang, Runqiu
Source
Journal of Immunology Research
Issue
Vol. 2011, Issue 2011 (31 Dec. 2010), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-28
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Transduction of latent membrane protein 2 (LMP2)-specific T-cell receptors into activated T lymphocytes may provide a universal, MHC-restricted mean to treat EBV-associated tumors in adoptive immunotherapy.
We compared TCR-specific promoters of distinct origin in lentiviral vectors, that is, Vβ6.7, delta, luria, and Vβ5.1 to evaluate TCR gene expression in human primary peripheral blood monocytes and T cell line HSB2.
Vectors containing Vβ 6.7 promoter were found to be optimal for expression in PBMCs, and they maintained expression of the transduced TCRs for up to 7 weeks.
These cells had the potential to recognize subdominant EBV latency antigens as measured by cytotoxicity and IFN-γ secretion.
The nude mice also exhibited significant resistance to the HLA-A2 and LMP2-positive CNE tumor cell challenge after being infused with lentiviral transduced CTLs.
In conclusion, LMP2-specific CTLs by lentiviral transduction have the potential use for treatment of EBV-related tumors.
American Psychological Association (APA)
Yang, Dongchang& Shao, Qing& Sun, Hua& Mu, Xiaoxin& Gao, Yun& Jiang, Runqiu…[et al.]. 2011. Evaluation of Epstein-Barr Virus Latent Membrane Protein 2 Specific T-Cell Receptors Driven by T-Cell Specific Promoters Using Lentiviral Vector. Journal of Immunology Research،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-990850
Modern Language Association (MLA)
Yang, Dongchang…[et al.]. Evaluation of Epstein-Barr Virus Latent Membrane Protein 2 Specific T-Cell Receptors Driven by T-Cell Specific Promoters Using Lentiviral Vector. Journal of Immunology Research Vol. 2011, no. 2011 (2010), pp.1-10.
https://search.emarefa.net/detail/BIM-990850
American Medical Association (AMA)
Yang, Dongchang& Shao, Qing& Sun, Hua& Mu, Xiaoxin& Gao, Yun& Jiang, Runqiu…[et al.]. Evaluation of Epstein-Barr Virus Latent Membrane Protein 2 Specific T-Cell Receptors Driven by T-Cell Specific Promoters Using Lentiviral Vector. Journal of Immunology Research. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-990850
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990850