Recombinant HBHA Boosting Effect on BCG-Induced Immunity against Mycobacterium tuberculosis Infection
Joint Authors
Source
Journal of Immunology Research
Issue
Vol. 2011, Issue 2011 (31 Dec. 2010), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-23
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Heterologous prime-boost regimens are effective strategies to promote long-term memory and strong cellular Th1 responses to Mycobacterium tuberculosis, when BCG is used in the priming step.
Subcutaneous or intranasal boosting of BCG-vaccinated newborn mice with native heparin-binding haemagglutinin (nHBHA) significantly enhances protection against M.
tuberculosis.
However, nHBHA is characterized by a complex methylation pattern in its C-terminal domain, which is important for protective immunogenicity in primary vaccination.
In this study we addressed the question whether boosting with recombinant, non-methylated HBHA (rHBHA) produced in Escherichia coli may enhance protection of BCG-primed newborn mice.
We found that while subcutaneous rHBHA boosting enhanced protection of BCG-primed mice against intranasal M.
tuberculosis infection both in spleen and lungs, enhanced protection against aerosol infection was only seen in the spleen (0.72 logs; P<0.05) but not in the lungs.
Thus, in BCG-primed mice the methylation of the C-terminal domain of HBHA is dispensable for the induction of enhanced protection in the lungs against intranasal but not aerosol infection, whereas it enhances protection in the spleen in both challenge models.
This report thus provides evidence that rHBHA may be considered as a booster vaccine against disseminated tuberculosis.
American Psychological Association (APA)
Guerrero, G. G.& Locht, Camille. 2011. Recombinant HBHA Boosting Effect on BCG-Induced Immunity against Mycobacterium tuberculosis Infection. Journal of Immunology Research،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-990853
Modern Language Association (MLA)
Guerrero, G. G.& Locht, Camille. Recombinant HBHA Boosting Effect on BCG-Induced Immunity against Mycobacterium tuberculosis Infection. Journal of Immunology Research Vol. 2011, no. 2011 (2010), pp.1-8.
https://search.emarefa.net/detail/BIM-990853
American Medical Association (AMA)
Guerrero, G. G.& Locht, Camille. Recombinant HBHA Boosting Effect on BCG-Induced Immunity against Mycobacterium tuberculosis Infection. Journal of Immunology Research. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-990853
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990853