Tumor and Microenvironment Modification during Progression of Murine Orthotopic Bladder Cancer
Joint Authors
Mahendran, Ratha
Tham, Sin Mun
Esuvaranathan, Kesavan
Pook, Sim Hwee
Ng, Kee Hui
Source
Journal of Immunology Research
Issue
Vol. 2011, Issue 2011 (31 Dec. 2010), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-13
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The aim of this study was to monitor changes in the expression of immune-related genes in the bladder after tumor implantation.
Mice were orthotopically implanted with MB49-PSA cells (C57BL/6 mice) on day 1 and terminated on days 7, 14, 21, and 28.
Another mouse model (MBT-2/C3H mice) was examined at day 7.
Gene expression analysis was performed using a TaqMan Low Density Mouse Immune Panel (Applied Biosystems, USA) on RNA extracted from the bladders.
Selected genes were reconfirmed by real-time PCR analysis and RT-PCR on the mRNA from other animals.
Immune suppressive (IL13, IL1β, PTGS2, NOS2, IL10, CTLA4, and CCL22) and immune stimulatory genes (CSF2, GZMB, IFNγ, CXCL10, TNFα, CD80, IL12a, and IL6) and AGTR2 were increased by day 7.
By day 28, IL10, CCL2, CCL5, CXCL11, CTLA4, GZMB, IFNγ, CSF2, and IL6 were significantly increased.
Therapeutic strategies involving TH1 induction and TH2 dampening may improve responses to immunotherapy.
American Psychological Association (APA)
Tham, Sin Mun& Ng, Kee Hui& Pook, Sim Hwee& Esuvaranathan, Kesavan& Mahendran, Ratha. 2011. Tumor and Microenvironment Modification during Progression of Murine Orthotopic Bladder Cancer. Journal of Immunology Research،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-990871
Modern Language Association (MLA)
Tham, Sin Mun…[et al.]. Tumor and Microenvironment Modification during Progression of Murine Orthotopic Bladder Cancer. Journal of Immunology Research Vol. 2011, no. 2011 (2010), pp.1-11.
https://search.emarefa.net/detail/BIM-990871
American Medical Association (AMA)
Tham, Sin Mun& Ng, Kee Hui& Pook, Sim Hwee& Esuvaranathan, Kesavan& Mahendran, Ratha. Tumor and Microenvironment Modification during Progression of Murine Orthotopic Bladder Cancer. Journal of Immunology Research. 2011. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-990871
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990871