Tyrosine Phosphorylation in the C-Terminal Nuclear Localization and Retention Signal (C-NLS) of the EWS Protein
Joint Authors
Leemann-Zakaryan, Ruzanna P.
Grossenbacher, Doris
Pahlich, Steffen
Gehring, Heinz
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-04-11
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Ewing sarcoma (EWS) proto-oncoprotein, an RNA-binding protein, is involved in DNA recombination and repair, gene expression, RNA processing and transport, as well as cell signalling.
Chimeric EWS oncoproteins generated by chromosomal translocations between EWSR1 and the genes of transcription factors cause malignant tumors.
To understand the loss of function by these translocations, the role of the intact EWS protein has to be investigated.
The predominantly nuclear localization of the EWS protein via a transportin-1-mediated mechanism is dependent on the recently identified C-NLS (also known as PY-NLS).
Among other residues in the C-NLS, Y656 interacts with transportin-1 and is essential for its nuclear localization.
Here, we show that Y656 is phosphorylated, which seems to be a critical factor for transportin-1-mediated nuclear import.
If Y656 was mutated cytosolic aggregates of the EWS protein, colocalized with transportin-1, were observed, similar to those described with mutants of the closely related FUS/TLS protein that had amino acid substitutions in the PY-NLS causing familial amyothrophic lateral sclerosis.
American Psychological Association (APA)
Leemann-Zakaryan, Ruzanna P.& Pahlich, Steffen& Grossenbacher, Doris& Gehring, Heinz. 2011. Tyrosine Phosphorylation in the C-Terminal Nuclear Localization and Retention Signal (C-NLS) of the EWS Protein. Complexity،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-991155
Modern Language Association (MLA)
Leemann-Zakaryan, Ruzanna P.…[et al.]. Tyrosine Phosphorylation in the C-Terminal Nuclear Localization and Retention Signal (C-NLS) of the EWS Protein. Complexity No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-991155
American Medical Association (AMA)
Leemann-Zakaryan, Ruzanna P.& Pahlich, Steffen& Grossenbacher, Doris& Gehring, Heinz. Tyrosine Phosphorylation in the C-Terminal Nuclear Localization and Retention Signal (C-NLS) of the EWS Protein. Complexity. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-991155
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991155