Impairment of Methotrexate Transport Is Common in Osteosarcoma Tumor Samples
Joint Authors
Richardson, Condon
Levy, Adam S.
Sowers, Rebecca
Meyers, Paul A.
Gorlick, Richard G.
Wenzel, Bethanne D.
Healey, John H.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-12-02
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Osteosarcoma does not respond well to conventional dose methotrexate but does respond to high-dose methotrexate.
Previous work has indicated that this resistance may be due to impaired transport of methotrexate across the cell membrane.
In this study, the PT430 competitive displacement assay was adapted to evaluate methotrexate transport in 69 high-grade osteosarcoma tumor samples.
All samples studied were shown to have relatively impaired methotrexate transport by PT430 assay.
Ninety-nine percent of the samples had less than 20% PT430 displacement by methotrexate.
Eighty-eight percent exhibited displacement by methotrexate at less than 50% of the displacement by trimetrexate.
The high frequency of impaired transport suggests the presence of decreased functionality of the reduced folate carrier protein.
The overwhelming presence of impaired transport may explain why methotrexate needs to be given in high doses to be effective in osteosarcoma therapy and suggests that reduced folate carrier-independent antifolates should be explored.
American Psychological Association (APA)
Sowers, Rebecca& Wenzel, Bethanne D.& Richardson, Condon& Meyers, Paul A.& Healey, John H.& Levy, Adam S.…[et al.]. 2010. Impairment of Methotrexate Transport Is Common in Osteosarcoma Tumor Samples. Complexity،Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-991216
Modern Language Association (MLA)
Sowers, Rebecca…[et al.]. Impairment of Methotrexate Transport Is Common in Osteosarcoma Tumor Samples. Complexity No. 2011 (2011), pp.1-5.
https://search.emarefa.net/detail/BIM-991216
American Medical Association (AMA)
Sowers, Rebecca& Wenzel, Bethanne D.& Richardson, Condon& Meyers, Paul A.& Healey, John H.& Levy, Adam S.…[et al.]. Impairment of Methotrexate Transport Is Common in Osteosarcoma Tumor Samples. Complexity. 2010. Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-991216
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991216