Involvement of the Soluble Urokinase Receptor in Chondrosarcoma Cell Mobilization
Joint Authors
La Rocca, Antonello
Di Carluccio, Gioconda
Fazioli, Flavio
De Chiara, Annarosaria
Pirozzi, Giuseppe
Rocco, Gaetano
Apice, Gaetano
Longanesi-Cattani, Immacolata
Gallo, Michele
Masucci, Maria Teresa
Bifulco, Katia
Carriero, Maria Vincenza
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-12-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
High levels of urokinase receptor (uPAR) in tissue and serum of patients with chondrosarcoma correlate with poor prognosis.
First, we analyzed the uPAR levels in tissues and plasma of five patients affected by chondrosarcoma.
Interestingly, very high levels of uPAR and its soluble forms (SuPAR) were found on tumor cell surfaces and plasma, respectively, of two patients with lung metastases.
Therefore, to investigate the role of SuPAR in chondrosaromas, we generated a primary cell culture from a chondrosarcoma tissue overexpressing uPAR on cell surfaces.
We found that chondrosarcoma-like primary culture cells release a large amount of SuPAR in the medium.
In vitro, SuPAR elicits chondrosarcoma cell migration likely through its uPAR88-92 sequence, since the DII88-183 or DIIDIIR88-284 uPAR domains retain motogen effect whereas DI1-87 or DIII184-284 domains, both lacking the uPAR88-92 sequence, are ineffective.
Chondrosarcoma cells cross matrigel in response to SuPAR, and their invasion capability is abrogated by RERF peptide which inhibits uPAR88-92 signalling.
These findings assign a role to uPAR in mobilizing chondrosarcoma cells and suggest that RERF peptide may be regarded as a prototype to generate new therapeutics for the chondrosarcoma treatment.
American Psychological Association (APA)
Bifulco, Katia& Longanesi-Cattani, Immacolata& Masucci, Maria Teresa& De Chiara, Annarosaria& Fazioli, Flavio& Di Carluccio, Gioconda…[et al.]. 2010. Involvement of the Soluble Urokinase Receptor in Chondrosarcoma Cell Mobilization. Complexity،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-991218
Modern Language Association (MLA)
Bifulco, Katia…[et al.]. Involvement of the Soluble Urokinase Receptor in Chondrosarcoma Cell Mobilization. Complexity No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-991218
American Medical Association (AMA)
Bifulco, Katia& Longanesi-Cattani, Immacolata& Masucci, Maria Teresa& De Chiara, Annarosaria& Fazioli, Flavio& Di Carluccio, Gioconda…[et al.]. Involvement of the Soluble Urokinase Receptor in Chondrosarcoma Cell Mobilization. Complexity. 2010. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-991218
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991218