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The Real-Time-Based Assessment of the Microbial Killing by the Antimicrobial Compounds of Neutrophils
Joint Authors
Lilius, Esa-Matti
Atosuo, J. T.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-05
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
A recombinant Escherichia coli K-12 strain, transformed with a modified bacterial luciferase gene (luxABCDE) from Photorhabdus luminescens, was constructed in order to monitor the activity of various antimicrobial agents on a real-time basis.
This E.
coli-lux emitted, without any addition of substrate, constitutive bioluminescence (BL), which correlated to the number of viable bacterial cells.
The decrease in BL signal correlated to the number of killed bacterial cells.
Antimicrobial activity of hydrogen peroxide (H2O2) and myeloperoxidase (MPO) was assessed.
In high concentrations, H2O2 alone had a bacteriocidic function and MPO enhanced this killing by forming hypochlorous acid (HOCl).
Taurine, the known HOCl scavenger, blocked the killing by MPO.
When E.
coli-lux was incubated with neutrophils, similar killing kinetics was recorded as in H2O2/MPO experiments.
The opsonization of bacteria enhanced the killing, and the maximum rate of the MPO release from lysosomes coincided with the onset of the killing.
American Psychological Association (APA)
Atosuo, J. T.& Lilius, Esa-Matti. 2011. The Real-Time-Based Assessment of the Microbial Killing by the Antimicrobial Compounds of Neutrophils. The Scientific World Journal،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-991297
Modern Language Association (MLA)
Atosuo, J. T.& Lilius, Esa-Matti. The Real-Time-Based Assessment of the Microbial Killing by the Antimicrobial Compounds of Neutrophils. The Scientific World Journal No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-991297
American Medical Association (AMA)
Atosuo, J. T.& Lilius, Esa-Matti. The Real-Time-Based Assessment of the Microbial Killing by the Antimicrobial Compounds of Neutrophils. The Scientific World Journal. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-991297
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991297