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Factors Affecting Poly(3-hydroxybutyrate) Production from Oil Palm Frond Juice by Cupriavidus necator (CCUG52238T)
Joint Authors
Salihon, Jailani
Mohd Zahari, Mior Ahmad Khushairi
Ariffin, Hidayah
Shirai, Yoshihito
Mokhtar, Mohd Noriznan
Hassan, Mohd Ali
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-08-21
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Factors influencing poly(3-hydroxybutyrate) P(3HB) production by Cupriavidus necator CCUG52238T utilizing oil palm frond (OPF) juice were clarified in this study.
Effects of initial medium pH, agitation speed, and ammonium sulfate (NH4)2SO4 concentration on the production of P(3HB) were investigated in shake flasks experiments using OPF juice as the sole carbon source.
The highest P(3HB) content was recorded at pH 7.0, agitation speed of 220 rpm, and (NH4)2SO4 concentration at 0.5 g/L.
By culturing the wild-type strain of C.
necator under the aforementioned conditions, the cell dry weight (CDW) and P(3HB) content obtained were 9.31 ± 0.13 g/L and 45 ± 1.5 wt.%, respectively.
This accounted for 40% increment of P(3HB) content compared to the nonoptimized condition.
In the meanwhile, the effect of dissolved oxygen tension (DOT) on P(3HB) production was investigated in a 2-L bioreactor.
Highest CDW (11.37 g/L) and P(3HB) content (44 wt.%) were achieved when DOT level was set at 30%.
P(3HB) produced from OPF juice had a tensile strength of 40 MPa and elongation at break of 8% demonstrated that P(3HB) produced from renewable and cheap carbon source is comparable to those produced from commercial substrate.
American Psychological Association (APA)
Mohd Zahari, Mior Ahmad Khushairi& Ariffin, Hidayah& Mokhtar, Mohd Noriznan& Salihon, Jailani& Shirai, Yoshihito& Hassan, Mohd Ali. 2012. Factors Affecting Poly(3-hydroxybutyrate) Production from Oil Palm Frond Juice by Cupriavidus necator (CCUG52238T). BioMed Research International،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991457
Modern Language Association (MLA)
Mohd Zahari, Mior Ahmad Khushairi…[et al.]. Factors Affecting Poly(3-hydroxybutyrate) Production from Oil Palm Frond Juice by Cupriavidus necator (CCUG52238T). BioMed Research International No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-991457
American Medical Association (AMA)
Mohd Zahari, Mior Ahmad Khushairi& Ariffin, Hidayah& Mokhtar, Mohd Noriznan& Salihon, Jailani& Shirai, Yoshihito& Hassan, Mohd Ali. Factors Affecting Poly(3-hydroxybutyrate) Production from Oil Palm Frond Juice by Cupriavidus necator (CCUG52238T). BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991457
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991457