Stable Plastid Transformation for High-Level Recombinant Protein Expression: Promises and Challenges
Joint Authors
Wang, Xianfeng
Li, Yongfei
Gao, Mei-li
Xue, Xiao-chang
Long, Jiangang
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-08
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Plants are a promising expression system for the production of recombinant proteins.
However, low protein productivity remains a major obstacle that limits extensive commercialization of whole plant and plant cell bioproduction platform.
Plastid genetic engineering offers several advantages, including high levels of transgenic expression, transgenic containment via maternal inheritance, and multigene expression in a single transformation event.
In recent years, the development of optimized expression strategies has given a huge boost to the exploitation of plastids in molecular farming.
The driving forces behind the high expression level of plastid bioreactors include codon optimization, promoters and UTRs, genotypic modifications, endogenous enhancer and regulatory elements, posttranslational modification, and proteolysis.
Exciting progress of the high expression level has been made with the plastid-based production of two particularly important classes of pharmaceuticals: vaccine antigens, therapeutic proteins, and antibiotics and enzymes.
Approaches to overcome and solve the associated challenges of this culture system that include low transformation frequencies, the formation of inclusion bodies, and purification of recombinant proteins will also be discussed.
American Psychological Association (APA)
Gao, Mei-li& Li, Yongfei& Xue, Xiao-chang& Wang, Xianfeng& Long, Jiangang. 2012. Stable Plastid Transformation for High-Level Recombinant Protein Expression: Promises and Challenges. BioMed Research International،Vol. 2012, no. 2012, pp.1-16.
https://search.emarefa.net/detail/BIM-991473
Modern Language Association (MLA)
Gao, Mei-li…[et al.]. Stable Plastid Transformation for High-Level Recombinant Protein Expression: Promises and Challenges. BioMed Research International No. 2012 (2012), pp.1-16.
https://search.emarefa.net/detail/BIM-991473
American Medical Association (AMA)
Gao, Mei-li& Li, Yongfei& Xue, Xiao-chang& Wang, Xianfeng& Long, Jiangang. Stable Plastid Transformation for High-Level Recombinant Protein Expression: Promises and Challenges. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-16.
https://search.emarefa.net/detail/BIM-991473
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991473