The Serine Protease Plasmin Triggers Expression of the CC-Chemokine Ligand 20 in Dendritic Cells via AktNF-κB-Dependent Pathways
Joint Authors
Syrovets, Tatiana
Simmet, Thomas
Li, Xuehua
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The number of dendritic cells is increased in advanced atherosclerotic lesions.
In addition, plasmin, which might stimulate dendritic cells, is generated in atherosclerotic lesions.
Here, we investigated cytokine and chemokine induction by plasmin in human dendritic cells.
In human atherosclerotic vessel sections, plasmin colocalized with dendritic cells and the CC-chemokine ligand 20 (CCL20, MIP-3α), which is important for homing of lymphocytes and dendritic cells to sites of inflammation.
Stimulation of human dendritic cells with plasmin, but not with catalytically inactivated plasmin, induced transcriptional regulation of CCL20.
By contrast, proinflammatory cytokines such as TNF-α, IL-1α, and IL-1β were not induced.
The plasmin-mediated CCL20 expression was preceded by activation of Akt and MAP kinases followed by activation of the transcription factor NF-κB as shown by phosphorylation of its inhibitor IκBα, by nuclear localization of p65, its phosphorylation, and binding to NF-κB consensus sequences.
The plasmin-induced CCL20 expression was dependent on Akt- and ERK1/2-mediated phosphorylation of IκBα on Ser32/36 and of p65 on Ser276, whereas p38 MAPK appeared to be dispensable.
Thus, plasmin triggers release of the chemokine CCL20 from dendritic cells, which might facilitate accumulation of CCR6+ immune cells in areas of plasmin generation such as inflamed tissues including atherosclerotic lesions.
American Psychological Association (APA)
Li, Xuehua& Syrovets, Tatiana& Simmet, Thomas. 2012. The Serine Protease Plasmin Triggers Expression of the CC-Chemokine Ligand 20 in Dendritic Cells via AktNF-κB-Dependent Pathways. BioMed Research International،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-991489
Modern Language Association (MLA)
Li, Xuehua…[et al.]. The Serine Protease Plasmin Triggers Expression of the CC-Chemokine Ligand 20 in Dendritic Cells via AktNF-κB-Dependent Pathways. BioMed Research International No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-991489
American Medical Association (AMA)
Li, Xuehua& Syrovets, Tatiana& Simmet, Thomas. The Serine Protease Plasmin Triggers Expression of the CC-Chemokine Ligand 20 in Dendritic Cells via AktNF-κB-Dependent Pathways. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-991489
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991489