Protegrin-1 Inhibits Dengue NS2B-NS3 Serine Protease and Viral Replication in MK2 Cells
Joint Authors
Yusof, Rohana
Sasikumer, Pottayil G.
Rothan, Hussin A.
Abdulrahman, Ammar Y.
Othman, Shatrah
Abd Rahman, Noorsaadah
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-02
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Dengue diseases have an economic as well as social burden worldwide.
In this study, the antiviral activity of protegrin-1 (PG-1, RGGRLCYCRRRFCVCVGR) peptide towards dengue NS2B-NS3pro and viral replication in Rhesus monkey kidney (MK2) cells was investigated.
The peptide PG-1 was synthesized by solid-phase peptide synthesis, and disulphide bonds formation followed by peptide purification was confirmed by LC-MS and RPHPLC.
Dengue NS2B-NS3pro was produced as a single-chain recombinant protein in E.
coli.
The NS2B-NS3pro assay was carried out by measuring the florescence emission of catalyzed substrate.
Real-time PCR was used to evaluate the inhibition potential of PG-1 towards dengue serotype-2 (DENV-2) replication in MK2 cells.
The results showed that PG-1 inhibited dengue NS2B-NS3pro at IC50 of 11.7 μM.
The graded concentrations of PG-1 at nontoxic range were able to reduce viral replication significantly (P<0.001) at 24, 48, and 72 hrs after viral infection.
However, the percentage of inhibition was significantly (P<0.01) higher at 24 hrs compared to 48 and 72 hrs.
These data show promising therapeutic potential of PG-1 against dengue infection, hence it warrants further analysis and improvement of the peptide features as a prospective starting point for consideration in designing attractive dengue virus inhibitors.
American Psychological Association (APA)
Rothan, Hussin A.& Abdulrahman, Ammar Y.& Sasikumer, Pottayil G.& Othman, Shatrah& Abd Rahman, Noorsaadah& Yusof, Rohana. 2012. Protegrin-1 Inhibits Dengue NS2B-NS3 Serine Protease and Viral Replication in MK2 Cells. BioMed Research International،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-991516
Modern Language Association (MLA)
Rothan, Hussin A.…[et al.]. Protegrin-1 Inhibits Dengue NS2B-NS3 Serine Protease and Viral Replication in MK2 Cells. BioMed Research International No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-991516
American Medical Association (AMA)
Rothan, Hussin A.& Abdulrahman, Ammar Y.& Sasikumer, Pottayil G.& Othman, Shatrah& Abd Rahman, Noorsaadah& Yusof, Rohana. Protegrin-1 Inhibits Dengue NS2B-NS3 Serine Protease and Viral Replication in MK2 Cells. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-991516
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991516