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Attenuated Salmonella typhimurium SV4089 as a Potential Carrier ofOral DNA Vaccine in Chickens
Joint Authors
Omar, Abdul Rahman
Zakaria, Zunita
Jazayeri, Seyed Davoud
Ideris, Aini
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-04
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Attenuated Salmonella has been used as a carrier for DNA vaccine.
However, in vitro and in vivo studies on the bacteria following transfection of plasmid DNA were poorly studied.
In this paper, eukaryotic expression plasmids encoding avian influenza virus (AIV) subtype H5N1 genes, pcDNA3.1/HA, NA, and NP, were transfected into an attenuated Salmonella enteric typhimurium SV4089.
In vitro stability of the transfected plasmids into Salmonella were over 90% after 100 generations.
The attenuated Salmonella were able to invade MCF-7 (1.2%) and MCF-10A (0.5%) human breast cancer cells.
Newly hatched specific-pathogen-free (SPF) chicks were inoculated once by oral gavage with 109 colony-forming unit (CFU) of the attenuated Salmonella.
No abnormal clinical signs or deaths were recorded after inoculation.
Viable bacteria were detected 3 days after inoculation by plating from spleen, liver, and cecum.
Fluorescent in situ hybridization (FISH) and polymerase chain reaction (PCR) were carried out for confirmation.
Salmonella was not detected in blood cultures although serum antibody immune responses to Salmonella O antiserum group D1 factor 1, 9, and 12 antigens were observed in all the inoculated chickens after 7 days up to 35 days.
Our results showed that live attenuated S.
typhimurium SV4089 harboring pcDNA3.1/HA, NA, and NP may provide a unique alternative as a carrier for DNA oral vaccine in chickens.
American Psychological Association (APA)
Jazayeri, Seyed Davoud& Ideris, Aini& Zakaria, Zunita& Omar, Abdul Rahman. 2012. Attenuated Salmonella typhimurium SV4089 as a Potential Carrier ofOral DNA Vaccine in Chickens. BioMed Research International،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991609
Modern Language Association (MLA)
Jazayeri, Seyed Davoud…[et al.]. Attenuated Salmonella typhimurium SV4089 as a Potential Carrier ofOral DNA Vaccine in Chickens. BioMed Research International No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-991609
American Medical Association (AMA)
Jazayeri, Seyed Davoud& Ideris, Aini& Zakaria, Zunita& Omar, Abdul Rahman. Attenuated Salmonella typhimurium SV4089 as a Potential Carrier ofOral DNA Vaccine in Chickens. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991609
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991609