PEDF and VEGF-A Output from Human Retinal Pigment Epithelial CellsGrown on Novel Microcarriers
Joint Authors
Congrove, Nicole R.
McCourt, Alexander D.
Zhang, Shiling
McKay, Brian S.
Sherman, Scott J.
Falk, Torsten
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-02
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Human retinal pigment epithelial (hRPE) cells have been tested as a cell-based therapy for Parkinson’s disease but will require additional study before further clinical trials can be planned.
We now show that the long-term survival and neurotrophic potential of hRPE cells can be enhanced by the use of FDA-approved plastic-based microcarriers compared to a gelatin-based microcarrier as used in failed clinical trials.
The hRPE cells grown on these plastic-based microcarriers display several important characteristics of hRPE found in vivo: (1) characteristic morphological features, (2) accumulation of melanin pigment, and (3) high levels of production of the neurotrophic factors pigment epithelium-derived factor (PEDF) and vascular endothelial growth factor-A (VEGF-A).
Growth of hRPE cells on plastic-based microcarriers led to sustained levels (>1 ng/ml) of PEDF and VEGF-A in conditioned media for two months.
We also show that the expression of VEGF-A and PEDF is reciprocally regulated by activation of the GPR143 pathway.
GPR143 is activated by L-DOPA (1 μM) which decreased VEGF-A secretion as opposed to the previously reported increase in PEDF secretion.
The hRPE microcarriers are therefore novel candidate delivery systems for achieving long-term delivery of the neuroprotective factors PEDF and VEGF-A, which could have a value in neurodegenerative conditions such as Parkinson’s disease.
American Psychological Association (APA)
Falk, Torsten& Congrove, Nicole R.& Zhang, Shiling& McCourt, Alexander D.& Sherman, Scott J.& McKay, Brian S.. 2012. PEDF and VEGF-A Output from Human Retinal Pigment Epithelial CellsGrown on Novel Microcarriers. BioMed Research International،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991617
Modern Language Association (MLA)
Falk, Torsten…[et al.]. PEDF and VEGF-A Output from Human Retinal Pigment Epithelial CellsGrown on Novel Microcarriers. BioMed Research International No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-991617
American Medical Association (AMA)
Falk, Torsten& Congrove, Nicole R.& Zhang, Shiling& McCourt, Alexander D.& Sherman, Scott J.& McKay, Brian S.. PEDF and VEGF-A Output from Human Retinal Pigment Epithelial CellsGrown on Novel Microcarriers. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991617
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991617