A New Candidate Substrate for Cell-Matrix Adhesion Study: The Acellular Human Amniotic Matrix
Joint Authors
Guo, Qianchen
Xue, Yuan
Zhao, Huajian
Lu, Xuya
Zhao, Xiaotao
Zheng, Hong
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-02
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
In vivo adhesions between cells and the extracellular matrix play a crucial role in cell differentiation, proliferation, and migration as well as tissue remodeling.
Natural three-dimensional (3D) matrices, such as self-assembling matrices and Matrigel, have limitations in terms of their biomechanical properties.
Here, we present a simple method to produce an acellular human amniotic matrix (AHAM) with preserved biomechanical properties and a favorable adhesion potential.
On the stromal side of the AHAM, human foreskin fibroblasts (HFFs) attached and extended with bipolar spindle-shaped morphology proliferated to multilayer networks, invaded into the AHAM, and migrated in a straight line.
Moreover, αV integrin, paxillin, and fibronectin were observed to colocalize after 24 h of HFF culture on the stromal side of the AHAM.
Our results indicate that the AHAM may be an ideal candidate as a cell-matrix adhesion substrate to study cell adhesion and invasion as well as other functions in vitro under a tensile force that mimics the in vivo environment.
American Psychological Association (APA)
Guo, Qianchen& Lu, Xuya& Xue, Yuan& Zheng, Hong& Zhao, Xiaotao& Zhao, Huajian. 2012. A New Candidate Substrate for Cell-Matrix Adhesion Study: The Acellular Human Amniotic Matrix. BioMed Research International،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-991633
Modern Language Association (MLA)
Guo, Qianchen…[et al.]. A New Candidate Substrate for Cell-Matrix Adhesion Study: The Acellular Human Amniotic Matrix. BioMed Research International No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-991633
American Medical Association (AMA)
Guo, Qianchen& Lu, Xuya& Xue, Yuan& Zheng, Hong& Zhao, Xiaotao& Zhao, Huajian. A New Candidate Substrate for Cell-Matrix Adhesion Study: The Acellular Human Amniotic Matrix. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-991633
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991633