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Is BAC Transgenesis Obsolete? State of the Art in the Era of Designer Nucleases
Joint Authors
Buch, T.
Beil, J.
Fairbairn, L.
Pelczar, P.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-13
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
DNA constructs based on bacterial artificial chromosomes (BACs) are frequently used to generate transgenic animals as they reduce the influence of position effects and allow predictable expression patterns for genes whose regulatory sequences are not fully identified.
Despite these advantages BAC transgenics suffer from drawbacks such as complicated vector construction, low efficiency of transgenesis, and some remaining expression variegation.
The recent development of transcription activator-like effector nucleases (TALENs) and zinc finger nucleases (ZFNs) has resulted in new transgenic techniques which do not have the drawbacks associated with BAC transgenesis.
Initial reports indicate that such designer nucleases (DNs) allow the targeted insertion of transgenes into endogenous loci by direct injection of the targeting vector and mRNA/DNA encoding the predesigned nucleases into oocytes.
This results in the transgene being inserted at a specific locus in the mouse genome, thus circumventing the drawbacks associated with BAC transgenesis.
American Psychological Association (APA)
Beil, J.& Fairbairn, L.& Pelczar, P.& Buch, T.. 2012. Is BAC Transgenesis Obsolete? State of the Art in the Era of Designer Nucleases. BioMed Research International،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-991635
Modern Language Association (MLA)
Beil, J.…[et al.]. Is BAC Transgenesis Obsolete? State of the Art in the Era of Designer Nucleases. BioMed Research International No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-991635
American Medical Association (AMA)
Beil, J.& Fairbairn, L.& Pelczar, P.& Buch, T.. Is BAC Transgenesis Obsolete? State of the Art in the Era of Designer Nucleases. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-991635
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991635