In Vivo Testing of MicroRNA-Mediated Gene Knockdown in Zebrafish
Joint Authors
Lan, Chuan-Ching
Love, Donald R.
Skinner, Jonathan R.
Leong, Ivone Un San
Shelling, Andrew N.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-27
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The zebrafish (Danio rerio) has become an attractive model for human disease modeling as there are a large number of orthologous genes that encode similar proteins to those found in humans.
The number of tools available to manipulate the zebrafish genome is limited and many currently used techniques are only effective during early development (such as morpholino-based antisense technology) or it is phenotypically driven and does not offer targeted gene knockdown (such as chemical mutagenesis).
The use of RNA interference has been met with controversy as off-target effects can make interpreting phenotypic outcomes difficult; however, this has been resolved by creating zebrafish lines that contain stably integrated miRNA constructs that target the desired gene of interest.
In this study, we show that a commercially available miRNA vector system with a mouse-derived miRNA backbone is functional in zebrafish and is effective in causing eGFP knockdown in a transient in vivo eGFP sensor assay system.
We chose to apply this system to the knockdown of transcripts that are implicated in the human cardiac disorder, Long QT syndrome.
American Psychological Association (APA)
Leong, Ivone Un San& Lan, Chuan-Ching& Skinner, Jonathan R.& Shelling, Andrew N.& Love, Donald R.. 2012. In Vivo Testing of MicroRNA-Mediated Gene Knockdown in Zebrafish. BioMed Research International،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-991648
Modern Language Association (MLA)
Leong, Ivone Un San…[et al.]. In Vivo Testing of MicroRNA-Mediated Gene Knockdown in Zebrafish. BioMed Research International No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-991648
American Medical Association (AMA)
Leong, Ivone Un San& Lan, Chuan-Ching& Skinner, Jonathan R.& Shelling, Andrew N.& Love, Donald R.. In Vivo Testing of MicroRNA-Mediated Gene Knockdown in Zebrafish. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-991648
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991648