Control and Augmentation of Long-Term Plasmid Transgene Expression In Vivo in Murine Muscle Tissue and Ex Vivo in Patient MesenchymalTissue
Joint Authors
Rajendran, Simon
Collins, Sara A.
van Pijkeren, Jan P.
Casey, Garrett
Morrissey, David
Tangney, Mark
O'Sullivan, Gerald C.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-30
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Purpose.
In vivo gene therapy directed at tissues of mesenchymal origin could potentially augment healing.
We aimed to assess the duration and magnitude of transene expression in vivo in mice and ex vivo in human tissues.
Methods.
Using bioluminescence imaging, plasmid and adenoviral vector-based transgene expression in murine quadriceps in vivo was examined.
Temporal control was assessed using a doxycycline-inducible system.
An ex vivo model was developed and optimised using murine tissue, and applied in ex vivo human tissue.
Results.
In vivo plasmid-based transgene expression did not silence in murine muscle, unlike in liver.
Although maximum luciferase expression was higher in muscle with adenoviral delivery compared with plasmid, expression reduced over time.
The inducible promoter cassette successfully regulated gene expression with maximum levels a factor of 11 greater than baseline.
Expression was re-induced to a similar level on a temporal basis.
Luciferase expression was readily detected ex vivo in human muscle and tendon.
Conclusions.
Plasmid constructs resulted in long-term in vivo gene expression in skeletal muscle, in a controllable fashion utilising an inducible promoter in combination with oral agents.
Successful plasmid gene transfection in human ex vivo mesenchymal tissue was demonstrated for the first time.
American Psychological Association (APA)
Morrissey, David& van Pijkeren, Jan P.& Rajendran, Simon& Collins, Sara A.& Casey, Garrett& O'Sullivan, Gerald C.…[et al.]. 2012. Control and Augmentation of Long-Term Plasmid Transgene Expression In Vivo in Murine Muscle Tissue and Ex Vivo in Patient MesenchymalTissue. BioMed Research International،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-991671
Modern Language Association (MLA)
Morrissey, David…[et al.]. Control and Augmentation of Long-Term Plasmid Transgene Expression In Vivo in Murine Muscle Tissue and Ex Vivo in Patient MesenchymalTissue. BioMed Research International No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-991671
American Medical Association (AMA)
Morrissey, David& van Pijkeren, Jan P.& Rajendran, Simon& Collins, Sara A.& Casey, Garrett& O'Sullivan, Gerald C.…[et al.]. Control and Augmentation of Long-Term Plasmid Transgene Expression In Vivo in Murine Muscle Tissue and Ex Vivo in Patient MesenchymalTissue. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-991671
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991671