Bacterial Artificial Chromosome Clones of Viruses Comprising the Towne Cytomegalovirus Vaccine

Joint Authors

Habib, EL-Sayed E.
Davison, Andrew J.
McVoy, Michael A.
Adler, Stuart P.
Cui, Xiaohong
Smith, Larry

Source

BioMed Research International

Issue

Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2011-11-30

Country of Publication

Egypt

No. of Pages

8

Main Subjects

Medicine

Abstract EN

Bacterial artificial chromosome (BAC) clones have proven invaluable for genetic manipulation of herpesvirus genomes.

BAC cloning can also be useful for capturing representative genomes that comprise a viral stock or mixture.

The Towne live attenuated cytomegalovirus vaccine was developed in the 1970s by serial passage in cultured fibroblasts.

Although its safety, immunogenicity, and efficacy have been evaluated in nearly a thousand human subjects, the vaccine itself has been little studied.

Instead, genetic composition and in vitro growth properties have been inferred from studies of laboratory stocks that may not always accurately represent the viruses that comprise the vaccine.

Here we describe the use of BAC cloning to define the genotypic and phenotypic properties of viruses from the Towne vaccine.

Given the extensive safety history of the Towne vaccine, these BACs provide a logical starting point for the development of next-generation rationally engineered cytomegalovirus vaccines.

American Psychological Association (APA)

Cui, Xiaohong& Adler, Stuart P.& Davison, Andrew J.& Smith, Larry& Habib, EL-Sayed E.& McVoy, Michael A.. 2011. Bacterial Artificial Chromosome Clones of Viruses Comprising the Towne Cytomegalovirus Vaccine. BioMed Research International،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991697

Modern Language Association (MLA)

Cui, Xiaohong…[et al.]. Bacterial Artificial Chromosome Clones of Viruses Comprising the Towne Cytomegalovirus Vaccine. BioMed Research International No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-991697

American Medical Association (AMA)

Cui, Xiaohong& Adler, Stuart P.& Davison, Andrew J.& Smith, Larry& Habib, EL-Sayed E.& McVoy, Michael A.. Bacterial Artificial Chromosome Clones of Viruses Comprising the Towne Cytomegalovirus Vaccine. BioMed Research International. 2011. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991697

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-991697