Neisseria gonorrhoeae Pilus Attenuates Cytokine Response of Human Fallopian Tube Explants

Joint Authors

Morales, Francisco
Orihuela, Pedro
Christodoulides, Myron
Rodas, Paula I.
Velasquez, L. A.
García, Katherine
Cardenas, Hugo
Heckels, John E.

Source

BioMed Research International

Issue

Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2011-12-19

Country of Publication

Egypt

No. of Pages

7

Main Subjects

Medicine

Abstract EN

A role for pilus during attachment of Neisseria gonorrhoeae to epithelia of the female reproductive tract is currently assumed.

However, Pil− gonococci have been observed during infection of the reproductive tract, which prompted us to examine the effect of pili on the dynamics of infection and the inflammatory responses of mucosal explants of the human Fallopian tube.

Methods.

Mucosal explants were infected in vitro with Opa negative Pil− and Pil+ N.

gonorrhoeae strains.

Results.

Piliation enhanced gonococcal adherence to the epithelium within 3 h of infection (P<0.05) but thereafter did not offer advantage to gonococci to colonize the epithelial cell surface (P>0.05).

No differences were found between the strains in numbers of gonococci inside epithelial cells.

Pil− bacteria induced higher levels (P<0.05) of IL-1β, TNF-α, GM-CSF, MCP-1, and MIP-1β than Pil+ bacteria.

There were no differences between both strains in LOS pattern, and Pil expression did not change after coincubation with mucosal strips.

Conclusions.

Results show that gonococcal invasion of the human Fallopian tube can occur independently of pilus or Opa expression, and suggest that pilus, by inhibition of several key elements of the initial inflammatory response, facilitates sustained infection of this organ.

American Psychological Association (APA)

Velasquez, L. A.& García, Katherine& Morales, Francisco& Heckels, John E.& Orihuela, Pedro& Rodas, Paula I.…[et al.]. 2011. Neisseria gonorrhoeae Pilus Attenuates Cytokine Response of Human Fallopian Tube Explants. BioMed Research International،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-991729

Modern Language Association (MLA)

Velasquez, L. A.…[et al.]. Neisseria gonorrhoeae Pilus Attenuates Cytokine Response of Human Fallopian Tube Explants. BioMed Research International No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-991729

American Medical Association (AMA)

Velasquez, L. A.& García, Katherine& Morales, Francisco& Heckels, John E.& Orihuela, Pedro& Rodas, Paula I.…[et al.]. Neisseria gonorrhoeae Pilus Attenuates Cytokine Response of Human Fallopian Tube Explants. BioMed Research International. 2011. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-991729

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-991729