Detection of Target ssDNA Using a Microfabricated Hall Magnetometer with Correlated Optical Readout
Joint Authors
Field, Mark
Aledealat, Khaled
Hira, Steven M.
von Molnár, Stephan
Xiong, Peng
Chase, P. Bryant
Strouse, Geoffrey F.
Sullivan, Gerard J.
Chen, Kan-Sheng
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-13
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Sensing biological agents at the genomic level, while enhancing the response time for biodetection over commonly used, optics-based techniques such as nucleic acid microarrays or enzyme-linked immunosorbent assays (ELISAs), is an important criterion for new biosensors.
Here, we describe the successful detection of a 35-base, single-strand nucleic acid target by Hall-based magnetic transduction as a mimic for pathogenic DNA target detection.
The detection platform has low background, large signal amplification following target binding and can discriminate a single, 350 nm superparamagnetic bead labeled with DNA.
Detection of the target sequence was demonstrated at 364 pM (<2 target DNA strands per bead) target DNA in the presence of 36 μM nontarget (noncomplementary) DNA (<10 ppm target DNA) using optical microscopy detection on a GaAs Hall mimic.
The use of Hall magnetometers as magnetic transduction biosensors holds promise for multiplexing applications that can greatly improve point-of-care (POC) diagnostics and subsequent medical care.
American Psychological Association (APA)
Hira, Steven M.& Aledealat, Khaled& Chase, P. Bryant& Xiong, Peng& von Molnár, Stephan& Strouse, Geoffrey F.…[et al.]. 2012. Detection of Target ssDNA Using a Microfabricated Hall Magnetometer with Correlated Optical Readout. BioMed Research International،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-991732
Modern Language Association (MLA)
Chase, P. Bryant…[et al.]. Detection of Target ssDNA Using a Microfabricated Hall Magnetometer with Correlated Optical Readout. BioMed Research International No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-991732
American Medical Association (AMA)
Hira, Steven M.& Aledealat, Khaled& Chase, P. Bryant& Xiong, Peng& von Molnár, Stephan& Strouse, Geoffrey F.…[et al.]. Detection of Target ssDNA Using a Microfabricated Hall Magnetometer with Correlated Optical Readout. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-991732
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991732