Effects of Norepinephrine and Acetylcholine on the Development of Cultured Leydig Cells in Mice
Joint Authors
Zhong, Xiuhui
Li, Yurong
Huo, Shuying
Wu, Xianjun
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-02
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Few data have suggested how norepinephrine (NE) and acetylcholine (Ach) regulate the development of Leydig cells in mice at prepuberty, except for data indicating endocrine effects.
The present study aims to elucidate the roles of NE and Ach on the differentiation and proliferation of Leydig cells.
Firstly, the expression of adrenergic receptors and muscarinic acetylcholine receptors in Leydig cells was investigated.
It was found that adrenergic receptors (β1AR, β2AR, and α1D) and muscarinic acetylcholine receptors (M1 and M3) mRNA are expressed in adult Leydig cells.
Then, the effects of NE and Ach on the differentiation and proliferation of Leydig cells were analyzed.
The results showed that NE and Ach at 10 μM significantly increased the number of 3β-hydroxysteroid-dehydrogenase- (3β-HSD-) positive Leydig cells and improved the expression of proliferating cell nuclear antigen (PCNA) in Leydig cells on postnatal day (PD) 15 (P<0.05).
NE and Ach at 10 μM had no impact on the expression of PCNA mRNA (P>0.05), but reduced the expression of 3β-HSD mRNA in adult Leydig cells and a murine Leydig tumor cell line (MLTC-1) (P<0.05).
Therefore, a conclusion may be reached that NE and Ach participated in stimulating the development of Leydig cells in mice from prepuberty to adult stage.
American Psychological Association (APA)
Huo, Shuying& Zhong, Xiuhui& Wu, Xianjun& Li, Yurong. 2012. Effects of Norepinephrine and Acetylcholine on the Development of Cultured Leydig Cells in Mice. BioMed Research International،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991738
Modern Language Association (MLA)
Huo, Shuying…[et al.]. Effects of Norepinephrine and Acetylcholine on the Development of Cultured Leydig Cells in Mice. BioMed Research International No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-991738
American Medical Association (AMA)
Huo, Shuying& Zhong, Xiuhui& Wu, Xianjun& Li, Yurong. Effects of Norepinephrine and Acetylcholine on the Development of Cultured Leydig Cells in Mice. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991738
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991738