PHA Productivity and Yield of Ralstonia eutropha When Intermittently or Continuously Fed a Mixture of Short Chain Fatty Acids
Joint Authors
Gibbons, William R.
Muthukumarappan, Kasiviswanathan
Chakraborty, Panchali
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-14
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The research described in this present study was part of a larger effort focused on developing a dual substrate, dual fermentation process to produce Polyhydroxyalkanoate (PHA).
The focus of this study was developing and optimizing a strategy for feeding a mixture of SCFAs (simulated ARF) and maximizing PHA production in a cost-effective way.
Three different feeding strategies were examined in this study.
The substrate evaluated in this study for the growth phase of R.
eutropha was condensed corn solubles, a low-value byproduct of the dry-mill, corn ethanol industry.
The culture was grown to high cell densities in nitrogen-supplemented condensed corn solubles media in 5 L bioreactors.
The overall growth rate of R.
eutropha was 0.2 h−1.
The 20 mL ARF feeding every 3 h from 48 to 109 h strategy gave the best results in terms of PHA production.
PHA productivity (0.0697 g L−1 h−1), PHA concentration (8.37 g L−1), and PHA content (39.52%) were the highest when ARF was fed every 3 h for 61 h.
This study proved that condensed corn solubles can be potentially used as a growth medium to boost PHA production by R.
eutropha thus reducing the overall cost of biopolymer production.
American Psychological Association (APA)
Chakraborty, Panchali& Muthukumarappan, Kasiviswanathan& Gibbons, William R.. 2012. PHA Productivity and Yield of Ralstonia eutropha When Intermittently or Continuously Fed a Mixture of Short Chain Fatty Acids. BioMed Research International،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991741
Modern Language Association (MLA)
Chakraborty, Panchali…[et al.]. PHA Productivity and Yield of Ralstonia eutropha When Intermittently or Continuously Fed a Mixture of Short Chain Fatty Acids. BioMed Research International No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-991741
American Medical Association (AMA)
Chakraborty, Panchali& Muthukumarappan, Kasiviswanathan& Gibbons, William R.. PHA Productivity and Yield of Ralstonia eutropha When Intermittently or Continuously Fed a Mixture of Short Chain Fatty Acids. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991741
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991741