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Cigarette Smoke Extract Stimulates Rat Pulmonary Artery Smooth Muscle Cell Proliferation via PKC-PDGFB Signaling
Joint Authors
Nie, Xin
Zhang, Huan-ping
Xing, Ai-ping
Li, Yi
Xu, Jian-ying
Du, Yong-cheng
Hu, Xiao-yun
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-11
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Accumulating evidence suggests a direct role for cigarette smoke in pulmonary vascular remodeling, which contributes to the development of pulmonary hypertension.
However, the molecular mechanisms underlying this process remain poorly understood.
Platelet-derived growth factor (PDGF) is a potential mitogen and chemoattractant implicated in several biological processes, including cell survival, proliferation, and migration.
In this study, we investigated the effect of cigarette smoke extract (CSE) on cell proliferation of rat pulmonary artery smooth muscle cells (rPASMCs).
We found that stimulation of rPASMCs with CSE significantly increased cell proliferation and promoted cell cycle progression from G1 phase to the S and G2 phases.
CSE treatment also significantly upregulated the mRNA and protein levels of PDGFB and PDGFRβ.
Our study also revealed that Rottlerin, an inhibitor of PKCδ signaling, prevented CSE-induced cell proliferation, attenuated the increase of S and G2 phase populations induced by CSE treatment, and downregulated PDGFB and PDGFRβ mRNA and protein levels in rPASMCs exposed to CSE.
Collectively, our data demonstrated that CSE-induced cell proliferation of rPASMCs involved upregulation of the PKCδ-PDGFB pathway.
American Psychological Association (APA)
Xing, Ai-ping& Du, Yong-cheng& Hu, Xiao-yun& Xu, Jian-ying& Zhang, Huan-ping& Li, Yi…[et al.]. 2012. Cigarette Smoke Extract Stimulates Rat Pulmonary Artery Smooth Muscle Cell Proliferation via PKC-PDGFB Signaling. BioMed Research International،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-991758
Modern Language Association (MLA)
Xing, Ai-ping…[et al.]. Cigarette Smoke Extract Stimulates Rat Pulmonary Artery Smooth Muscle Cell Proliferation via PKC-PDGFB Signaling. BioMed Research International No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-991758
American Medical Association (AMA)
Xing, Ai-ping& Du, Yong-cheng& Hu, Xiao-yun& Xu, Jian-ying& Zhang, Huan-ping& Li, Yi…[et al.]. Cigarette Smoke Extract Stimulates Rat Pulmonary Artery Smooth Muscle Cell Proliferation via PKC-PDGFB Signaling. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-991758
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991758