Evaluation of Bovine Embryo Biopsy Techniques according to Their Ability to Preserve Embryo Viability
Joint Authors
Cernea, C.
Pall, E.
Groza, I.
Cenariu, M.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-02
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
The purpose of this research was to evaluate three embryo biopsy techniques used for preimplantation genetic diagnosis (PGD) in cattle and to recommend the least invasive one for current use, especially when PGD is followed by embryo cryopreservation.
Three hundred bovine embryos were biopsied by either one of the needle, aspiration or microblade method, and then checked for viability by freezing/thawing and transplantation to recipient cows.
The number of pregnancies obtained after the transfer of biopsied frozen/thawed embryos was assessed 30 days later using ultrasounds.
The results were significantly different between the three biopsy methods: the pregnancy rate was of 57% in cows that received embryos biopsied by needle, 43% in cows that received embryos biopsied by aspiration, and 31% in cows that received embryos biopsied by microblade.
Choosing an adequate biopsy method is therefore of great importance in embryos that will undergo subsequent cryopreservation, as it significantly influences their viability after thawing.
American Psychological Association (APA)
Cenariu, M.& Pall, E.& Cernea, C.& Groza, I.. 2012. Evaluation of Bovine Embryo Biopsy Techniques according to Their Ability to Preserve Embryo Viability. BioMed Research International،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-991764
Modern Language Association (MLA)
Cenariu, M.…[et al.]. Evaluation of Bovine Embryo Biopsy Techniques according to Their Ability to Preserve Embryo Viability. BioMed Research International No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-991764
American Medical Association (AMA)
Cenariu, M.& Pall, E.& Cernea, C.& Groza, I.. Evaluation of Bovine Embryo Biopsy Techniques according to Their Ability to Preserve Embryo Viability. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-991764
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991764