Structural Insights into Interaction between Mammalian Methionine Sulfoxide Reductase B1 and Thioredoxin
Joint Authors
Dikiy, Alexander
Dobrovolska, Olena
Shabalin, Konstantin
Nerinovski, Kirill
Rychkov, Georgy
Schmidt, Alexander
Shumilina, Elena
Yakimov, Alexander
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-13
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Maintenance of the cellular redox balance has vital importance for correcting organism functioning.
Methionine sulfoxide reductases (Msrs) are among the key members of the cellular antioxidant defence system.
To work properly, methionine sulfoxide reductases need to be reduced by their biological partner, thioredoxin (Trx).
This process, according to the available kinetic data, represents the slowest step in the Msrs catalytic cycle.
In the present paper, we investigated structural aspects of the intermolecular complex formation between mammalian MsrB1 and Trx.
NMR spectroscopy and biocomputing were the two mostly used through the research approaches.
The formation of NMR detectable MsrB1/Trx complex was monitored and studied in attempt to understand MsrB1 reduction mechanism.
Using NMR data, molecular mechanics, protein docking, and molecular dynamics simulations, it was found that intermediate MsrB1/Trx complex is stabilized by interprotein β-layer.
The complex formation accompanied by distortion of disulfide bond within MsrB1 facilitates the reduction of oxidized MsrB1 as it is evidenced by the obtained data.
American Psychological Association (APA)
Dobrovolska, Olena& Rychkov, Georgy& Shumilina, Elena& Nerinovski, Kirill& Schmidt, Alexander& Shabalin, Konstantin…[et al.]. 2012. Structural Insights into Interaction between Mammalian Methionine Sulfoxide Reductase B1 and Thioredoxin. BioMed Research International،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-991780
Modern Language Association (MLA)
Dobrovolska, Olena…[et al.]. Structural Insights into Interaction between Mammalian Methionine Sulfoxide Reductase B1 and Thioredoxin. BioMed Research International No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-991780
American Medical Association (AMA)
Dobrovolska, Olena& Rychkov, Georgy& Shumilina, Elena& Nerinovski, Kirill& Schmidt, Alexander& Shabalin, Konstantin…[et al.]. Structural Insights into Interaction between Mammalian Methionine Sulfoxide Reductase B1 and Thioredoxin. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-991780
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991780