Hydrogen Photoproduction by Rhodopseudomonas palustris 42OL Cultured at High Irradiance under a Semicontinuous Regime

Abstract EN

The main goal of this study was to increase the hydrogen production rate improving the culture technique and the photobioreactor performances.

Experiments were carried out at a constant culture temperature of 30°C and at an average irradiance of 480 W m−2 using a cylindrical photobioreactor (4.0 cm, internal diameter).

The culture technique, namely, the semicontinuous regime for growing Rhodopseudomonas palustris 42OL made it possible to achieve a very high daily hydrogen production rate of 594 ± 61 mL (H2) L−1 d−1.

This value, never reported for this strain, corresponds to about 25 mL (H2) L−1 h−1, and it was obtained when the hydraulic retention time (HRT) was of 225 hours.

Under the same growth conditions, a very high biomass production rate (496 ± 45 mg (dw) L−1 d−1) was also achieved.

Higher or lower HRTs caused a reduction in both the hydrogen and the biomass production rates.

The malic-acid removal efficiency (MAre) was always higher than 90%.

The maximal hydrogen yield was 3.03 mol H2 mol MA−1 at the HRT of 360 hours.

The highest total energy conversion efficiency was achieved at the HRT of 225 hours.