Improved Production of Tryptophan in Genetically Engineered Escherichia coli with TktA and PpsA Overexpression
Joint Authors
Xie, Xixian
Chen, Ning
Liu, Qing
Shen, Tong
Xu, Qingyang
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-17
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Intracellular precursor supply is a critical factor for amino acid productivity.
In the present study, ppsA and tktA genes were overexpressed in genetically engineered Escherichia coli to enhance the availability of two precursor substrates, phosphoenolpyruvate and erythrose-4-phosphate.
The engineered strain, TRTH0709 carrying pSV709, produced 35.9 g/L tryptophan from glucose after 40 h in fed-batch cultivation.
The two genes were inserted, independently or together, into a low-copy-number expression vector (pSTV28) and transferred to TRTH0709.
Fed-batch fermentations at high cell densities of the recombination strains revealed that overexpression of the ppsA gene alone does not significantly increase tryptophan yield.
On the other hand, overexpression of the tktA gene, alone or with the ppsA gene, could further improve tryptophan yield to a final tryptophan titer of 37.9 and 40.2 g/L, respectively.
These results represent a 5.6% and 11.9% enhancement over the titer achieved by TRTH0709.
No evident genetic modifications leading to growth impairment were observed.
American Psychological Association (APA)
Shen, Tong& Liu, Qing& Xie, Xixian& Xu, Qingyang& Chen, Ning. 2012. Improved Production of Tryptophan in Genetically Engineered Escherichia coli with TktA and PpsA Overexpression. BioMed Research International،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991801
Modern Language Association (MLA)
Shen, Tong…[et al.]. Improved Production of Tryptophan in Genetically Engineered Escherichia coli with TktA and PpsA Overexpression. BioMed Research International No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-991801
American Medical Association (AMA)
Shen, Tong& Liu, Qing& Xie, Xixian& Xu, Qingyang& Chen, Ning. Improved Production of Tryptophan in Genetically Engineered Escherichia coli with TktA and PpsA Overexpression. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991801
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991801