Analyzing Gene Expression Profile in K562 Cells Exposed to Sodium Valproate Using Microarray Combined with the Connectivity Map Database
Joint Authors
Lin, Dong-Jun
Wang, Chun-Huai
Ding, Qian
Zhang, Xiang-Zhong
Zhu, Xiao-Yu
Yin, Ai-Hua
Chen, Yun-Xian
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-04
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
To explore the mechanism underlying antileukaemia effect of sodium valproate, the growth and survival of the K562 cell line were investigated.
Global profiles of gene expression in K562 cells exposed to sodium valproate were assessed and validated.
The differentially expressed genes identified were further used to query the connectivity map database to retrieve a ranked list of compounds that act on the same intracellular targets as sodium valproate.
A significant increase in cell apoptosis and a change in gene expression profile were observed in valproate-exposed K562 cells.
The significant enrichment analysis of gene ontology terms for the differentially expressed genes showed that these genes were involved in many important biological processes.
Eight differentially expressed genes involved in apoptosis were verified by quantitative real-time PCR.
The connectivity map analysis showed gene expression profile in K562 cells exposed to sodium valproate was most similar to that of HDACi and PI3K inhibitors, suggesting that sodium valproate might exert antileukaemic action by inhibiting HDAC as well as inhibiting PI3K pathway.
In conclusion, our data might provide clues to elucidate the molecular and therapeutic potential of VPA in leukaemia treatment, and the connectivity map is a useful tool for exploring the molecular mechanism of drug action.
American Psychological Association (APA)
Zhang, Xiang-Zhong& Yin, Ai-Hua& Lin, Dong-Jun& Zhu, Xiao-Yu& Ding, Qian& Wang, Chun-Huai…[et al.]. 2012. Analyzing Gene Expression Profile in K562 Cells Exposed to Sodium Valproate Using Microarray Combined with the Connectivity Map Database. BioMed Research International،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991826
Modern Language Association (MLA)
Zhang, Xiang-Zhong…[et al.]. Analyzing Gene Expression Profile in K562 Cells Exposed to Sodium Valproate Using Microarray Combined with the Connectivity Map Database. BioMed Research International No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-991826
American Medical Association (AMA)
Zhang, Xiang-Zhong& Yin, Ai-Hua& Lin, Dong-Jun& Zhu, Xiao-Yu& Ding, Qian& Wang, Chun-Huai…[et al.]. Analyzing Gene Expression Profile in K562 Cells Exposed to Sodium Valproate Using Microarray Combined with the Connectivity Map Database. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991826
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991826