Micromechanical Thermal Assays of Ca2+-Regulated Thin-Filament Function and Modulation by Hypertrophic Cardiomyopathy Mutants of Human Cardiac Troponin
Joint Authors
Mihajlović, Goran
von Molnár, Stephan
Wang, Fang
Xiong, Peng
Chase, P. Bryant
Brunet, Nicolas M.
Aledealat, Khaled
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-14
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Microfabricated thermoelectric controllers can be employed to investigate mechanisms underlying myosin-driven sliding of Ca2+-regulated actin and disease-associated mutations in myofilament proteins.
Specifically, we examined actin filament sliding—with or without human cardiac troponin (Tn) and α-tropomyosin (Tm)—propelled by rabbit skeletal heavy meromyosin, when temperature was varied continuously over a wide range (~20–63C°).
At the upper end of this temperature range, reversible dysregulation of thin filaments occurred at pCa 9 and 5; actomyosin function was unaffected.
Tn-Tm enhanced sliding speed at pCa 5 and increased a transition temperature (Tt) between a high activation energy (Ea) but low temperature regime and a low Ea but high temperature regime.
This was modulated by factors that alter cross-bridge number and kinetics.
Three familial hypertrophic cardiomyopathy (FHC) mutations, cTnI R145G, cTnI K206Q, and cTnT R278C, cause dysregulation at temperatures ~5–8C° lower; the latter two increased speed at pCa 5 at all temperatures.
American Psychological Association (APA)
Brunet, Nicolas M.& Mihajlović, Goran& Aledealat, Khaled& Wang, Fang& Xiong, Peng& von Molnár, Stephan…[et al.]. 2012. Micromechanical Thermal Assays of Ca2+-Regulated Thin-Filament Function and Modulation by Hypertrophic Cardiomyopathy Mutants of Human Cardiac Troponin. BioMed Research International،Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-991828
Modern Language Association (MLA)
Brunet, Nicolas M.…[et al.]. Micromechanical Thermal Assays of Ca2+-Regulated Thin-Filament Function and Modulation by Hypertrophic Cardiomyopathy Mutants of Human Cardiac Troponin. BioMed Research International No. 2012 (2012), pp.1-13.
https://search.emarefa.net/detail/BIM-991828
American Medical Association (AMA)
Brunet, Nicolas M.& Mihajlović, Goran& Aledealat, Khaled& Wang, Fang& Xiong, Peng& von Molnár, Stephan…[et al.]. Micromechanical Thermal Assays of Ca2+-Regulated Thin-Filament Function and Modulation by Hypertrophic Cardiomyopathy Mutants of Human Cardiac Troponin. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-991828
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991828