EhADH112 Is a Bro1 Domain-Containing Protein Involved in the Entamoeba histolytica Multivesicular Bodies Pathway
Joint Authors
López-Reyes, Israel
García-Rivera, Guillermina
González-Robles, Arturo
Mendoza, Leobardo
Bañuelos, Cecilia
Orozco, Esther
Vincent, Olivier
Herranz, Silvia
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-28
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
EhADH112 is an Entamoeba histolytica Bro1 domain-containing protein, structurally related to mammalian ALIX and yeast BRO1, both involved in the Endosomal Sorting Complexes Required for Transport (ESCRT)-mediated multivesicular bodies (MVB) biogenesis.
Here, we investigated an alternative role for EhADH112 in the MVB protein trafficking pathway by overexpressing 166 amino acids of its N-terminal Bro1 domain in trophozoites.
Trophozoites displayed diminished phagocytosis rates and accumulated exogenous Bro1 at cytoplasmic vesicles which aggregated into aberrant complexes at late stages of phagocytosis, probably preventing EhADH112 function.
Additionally, the existence of a putative E.
histolytica ESCRT-III subunit (EhVps32) presumably interacting with EhADH112, led us to perform pull-down experiments with GST-EhVps32 and [35S]-labeled EhADH112 or EhADH112 derivatives, confirming EhVps32 binding to EhADH112 through its Bro1 domain.
Our overall results define EhADH112 as a novel member of ESCRT-accessory proteins transiently present at cellular surface and endosomal compartments, probably contributing to MVB formation during phagocytosis.
American Psychological Association (APA)
Bañuelos, Cecilia& García-Rivera, Guillermina& López-Reyes, Israel& Mendoza, Leobardo& González-Robles, Arturo& Herranz, Silvia…[et al.]. 2012. EhADH112 Is a Bro1 Domain-Containing Protein Involved in the Entamoeba histolytica Multivesicular Bodies Pathway. BioMed Research International،Vol. 2012, no. 2012, pp.1-15.
https://search.emarefa.net/detail/BIM-991829
Modern Language Association (MLA)
Bañuelos, Cecilia…[et al.]. EhADH112 Is a Bro1 Domain-Containing Protein Involved in the Entamoeba histolytica Multivesicular Bodies Pathway. BioMed Research International No. 2012 (2012), pp.1-15.
https://search.emarefa.net/detail/BIM-991829
American Medical Association (AMA)
Bañuelos, Cecilia& García-Rivera, Guillermina& López-Reyes, Israel& Mendoza, Leobardo& González-Robles, Arturo& Herranz, Silvia…[et al.]. EhADH112 Is a Bro1 Domain-Containing Protein Involved in the Entamoeba histolytica Multivesicular Bodies Pathway. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-15.
https://search.emarefa.net/detail/BIM-991829
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991829