miRNA589 Regulates Epithelial-Mesenchymal Transition in Human Peritoneal Mesothelial Cells
Joint Authors
Peng, You-ming
Zhang, Ke
Zhou, Xun
Liu, Ying-hong
Xiao, Li
Tang, Wen-bin
Sun, Lin
Liu, Hong
Liu, Fu-you
Zhang, Hao
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-03
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
microRNA (miRNA, miR) are thought to interact with multiple mRNAs which are involved in the EMT process.
But the role of miRNAs in peritoneal fibrosis has remained unknown.
Objective.
To determine if miRNA589 regulates the EMT induced by TGFβ1 in human peritoneal mesothelial cell line (HMrSV5 cells).
Methods.
1.
Level of miR589 was detected in both human peritoneal mesothelial cells (HPMCs) isolated from continuous ambulatory peritoneal dialysis (CAPD) patients’ effluent and HMrSV5 cells treated with or without TGFβ1.
2.
HMrSV5 cells were divided into three groups: control group, TGFβ1 group, and pre-miR-589+TGFβ1 group.
The level of miRNA589 was determined by realtime PCR.
The expressions of ZO-1, vimentin, and E-cadherin in HPMCs were detected, respectively.
Results.
Decreased level of miRNA589 was obtained in either HPMCs of long-term CAPD patients or HMrSV5 cells treated with TGFβ1.
In vitro, TGFβ1 led to upregulation of vimentin and downregulation of ZO-1 as well as E-cadherin in HMrSV5 cells, which suggested EMT, was induced.
The changes were accompanied with notably decreased level of miRNA589 in HMrSV5 cells treated with TGFβ1.
Overexpression of miRNA589 by transfection with pre-miRNA589 partially reversed these EMT changes.
Conclusion.
miRNA589 mediates TGFβ1 induced EMT in human peritoneal mesothelial cells.
American Psychological Association (APA)
Zhang, Ke& Zhang, Hao& Zhou, Xun& Tang, Wen-bin& Xiao, Li& Liu, Ying-hong…[et al.]. 2012. miRNA589 Regulates Epithelial-Mesenchymal Transition in Human Peritoneal Mesothelial Cells. BioMed Research International،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-991835
Modern Language Association (MLA)
Zhang, Ke…[et al.]. miRNA589 Regulates Epithelial-Mesenchymal Transition in Human Peritoneal Mesothelial Cells. BioMed Research International No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-991835
American Medical Association (AMA)
Zhang, Ke& Zhang, Hao& Zhou, Xun& Tang, Wen-bin& Xiao, Li& Liu, Ying-hong…[et al.]. miRNA589 Regulates Epithelial-Mesenchymal Transition in Human Peritoneal Mesothelial Cells. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-991835
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991835