Proteomic Characterization of a Mouse Model of Familial Danish Dementia
Joint Authors
Zambrano, Nicola
D'Adamio, Luciano
Renzone, Giovanni
Matsuda, Shuji
Scaloni, Andrea
Vitale, Monica
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-26
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A dominant mutation in the ITM2B/BRI2 gene causes familial Danish dementia (FDD) in humans.
To model FDD in animal systems, a knock-in approach was recently implemented in mice expressing a wild-type and mutant allele, which bears the FDD-associated mutation.
Since these FDDKI mice show behavioural alterations and impaired synaptic function, we characterized their synaptosomal proteome via two-dimensional differential in-gel electrophoresis.
After identification by nanoliquid chromatography coupled to electrospray-linear ion trap tandem mass spectrometry, the differentially expressed proteins were classified according to their gene ontology descriptions and their predicted functional interactions.
The Dlg4/Psd95 scaffold protein and additional signalling proteins, including protein phosphatases, were revealed by STRING analysis as potential players in the altered synaptic function of FDDKI mice.
Immunoblotting analysis finally demonstrated the actual downregulation of the synaptosomal scaffold protein Dlg4/Psd95 and of the dual-specificity phosphatase Dusp3 in the synaptosomes of FDDKI mice.
American Psychological Association (APA)
Vitale, Monica& Renzone, Giovanni& Matsuda, Shuji& Scaloni, Andrea& D'Adamio, Luciano& Zambrano, Nicola. 2012. Proteomic Characterization of a Mouse Model of Familial Danish Dementia. BioMed Research International،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991858
Modern Language Association (MLA)
Vitale, Monica…[et al.]. Proteomic Characterization of a Mouse Model of Familial Danish Dementia. BioMed Research International No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-991858
American Medical Association (AMA)
Vitale, Monica& Renzone, Giovanni& Matsuda, Shuji& Scaloni, Andrea& D'Adamio, Luciano& Zambrano, Nicola. Proteomic Characterization of a Mouse Model of Familial Danish Dementia. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991858
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991858