Gecko Crude Peptides Induce Apoptosis in Human Liver Carcinoma Cells In Vitro and Exert Antitumor Activity in a Mouse Ascites H22 Xenograft Model
Joint Authors
Lu, Fei
Li, Yan
Cui, Zhao-Chu
Wang, Jian-Gang
Duan, Leng-Xin
Li, Rui-Fang
Song, Ying
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-03
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Aim.
To investigate the anti-tumor effects and mechanisms of gecko crude peptides (GCPs) in vitro and in vivo.
Methods.
3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay was applied to measure the effects of GCPs on the HepG2 cell viability.
Fluorescence morphology was used to identify apoptotic cells.
A xenograft H22 liver cancer model was established in Kunming mice.
The tumor-bearing mice were treated with daily intraperitoneal injections of normal saline (NS group) or GCPs (80, 40 or 20 mg/kg) for 10 days, or once per two days with 2 mg/kg doxorubicin (ADR group; n=10 each).
Serum tumor necrosis factor (TNF-α) and interleukin (IL)-6 were quantified using ELISA assay.
Results.
GCPs significantly inhibited the growth of HepG2 cells and induced typical apoptotic morphological features through increasing bcl-2/bax ratio in a dose- and time-dependent manner in vitro.
The tumor weights of the ADR group, GCPs (H) group, GCPs (M) group, GCPs (L) group were smaller compared to the NS group.
While the white blood cell count, thymus index, spleen index were higher in the high dose GCPs group than the NS group (P<0.05), the VEGF expression in tumor tissue and serum TNF-α and IL-6 levels in the GCPs groups were lower than the NS group (P<0.05).
American Psychological Association (APA)
Song, Ying& Wang, Jian-Gang& Li, Rui-Fang& Li, Yan& Cui, Zhao-Chu& Duan, Leng-Xin…[et al.]. 2012. Gecko Crude Peptides Induce Apoptosis in Human Liver Carcinoma Cells In Vitro and Exert Antitumor Activity in a Mouse Ascites H22 Xenograft Model. BioMed Research International،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-991874
Modern Language Association (MLA)
Song, Ying…[et al.]. Gecko Crude Peptides Induce Apoptosis in Human Liver Carcinoma Cells In Vitro and Exert Antitumor Activity in a Mouse Ascites H22 Xenograft Model. BioMed Research International No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-991874
American Medical Association (AMA)
Song, Ying& Wang, Jian-Gang& Li, Rui-Fang& Li, Yan& Cui, Zhao-Chu& Duan, Leng-Xin…[et al.]. Gecko Crude Peptides Induce Apoptosis in Human Liver Carcinoma Cells In Vitro and Exert Antitumor Activity in a Mouse Ascites H22 Xenograft Model. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-991874
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991874